OGS1158
PSF-CMV-PURO-COOH-EKT-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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0.1 MG
¥3,109.08
250 μG
¥3,812.01
¥3,109.08
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0.1 MG
¥3,109.08
250 μG
¥3,812.01
About This Item
UNSPSC代码:
12352200
NACRES:
NA.85
¥3,109.08
请联系客服了解存货情况
标签
10-His tagged
表单
buffered aqueous solution
分子量
size 6195 bp
菌种筛选
kanamycin
哺乳动物细胞筛选
puromycin
复制起点
pUC (500 copies)
肽切割
EKT
肽标签位置
C-terminal
启动子
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
报告基因
none
1 of 4
此商品 | OGS1157 | OGS1080 | OGS1119 |
|---|---|---|---|
| peptide tag location C-terminal | peptide tag location N-terminal | peptide tag location C-terminal | peptide tag location N-terminal |
| promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV |
| peptide cleavage EKT | peptide cleavage EKT | peptide cleavage thrombin | peptide cleavage TEV |
| mammalian cells selection puromycin | mammalian cells selection puromycin | mammalian cells selection puromycin | mammalian cells selection puromycin |
| bacteria selection kanamycin | bacteria selection kanamycin | bacteria selection kanamycin | bacteria selection kanamycin |
| origin of replication pUC (500 copies) | origin of replication pUC (500 copies) | origin of replication pUC (500 copies) | origin of replication pUC (500 copies) |
一般描述
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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