OGS1080
PSF-CMV-PURO-COOH-THR-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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10 G
¥3,405.04
¥3,405.04
国内现货，预计发货时间2025年5月06日详情
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10 G
¥3,405.04
About This Item
UNSPSC代码:
12352200
NACRES:
NA.85
¥3,405.04
国内现货，预计发货时间2025年5月06日详情
通知我
有国内现货时，请邮件通知我。
标签
10-His tagged
表单
buffered aqueous solution
分子量
size 6198 bp
菌种筛选
kanamycin
哺乳动物细胞筛选
puromycin
复制起点
pUC (500 copies)
肽切割
thrombin
肽标签位置
C-terminal
启动子
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
报告基因
none
1 of 4
此商品 | 69875 | 199958 | 800396 |
|---|---|---|---|
| assay ≥99.9% trace metals basis | assay - | assay 98% | assay - |
| form solid | form solid | form solid | form powder |
| mp 150 °C (dec.) (lit.) | mp 150 °C (dec.) (lit.) | mp 150 °C (dec.) (lit.) | mp 147-152 °C (decomposition) |
| density 1.96 g/mL at 25 °C (lit.) | density 1.96 g/mL at 25 °C (lit.) | density 1.96 g/mL at 25 °C (lit.) | density 1.96 g/cm3 at 20 °C |
| vapor density 9.1 (vs air) | vapor density 9.1 (vs air) | vapor density 9.1 (vs air) | vapor density - |
| bp 156 °C (lit.) | bp 156 °C (lit.) | bp 156 °C (lit.) | bp - |
一般描述
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a Thrombin cleavage tag. The protein sequence of the cleavage tag is: LVPRGS. It cleaves preferentially between the Arg and Gly residues. Off target cleavage can often occur at non-specific sites normally from other contaminating proteases. To ensure maximal protein integrity the enzyme reagent must be highly pure.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a Thrombin cleavage tag. The protein sequence of the cleavage tag is: LVPRGS. It cleaves preferentially between the Arg and Gly residues. Off target cleavage can often occur at non-specific sites normally from other contaminating proteases. To ensure maximal protein integrity the enzyme reagent must be highly pure.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
序列
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
分析说明
To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.
其他说明
Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.
法律信息
Oxford Genetics is a trademark of Oxford Genetics Ltd
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12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
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法规信息
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