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Merck
CN

O7505

地衣红

certified by the Biological Stain Commission, powder

别名:

天然红28

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关于此项目

化学文摘社编号:
UNSPSC Code:
12171500
NACRES:
NA.47
EC Number:
215-750-6
MDL number:
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产品名称

地衣红, synthetic, certified by the BSC

SMILES string

N(c5c(cc(cc5C)O)O)C1=C(c2[c]([o][c]3c2C(=C(C(=O)C=3)Nc4c(cc(cc4C)O)O)C)=CC1=O)C

InChI

1S/C28H24N2O7/c1-11-5-15(31)7-17(33)25(11)29-27-13(3)23-21(9-19(27)35)37-22-10-20(36)28(14(4)24(22)23)30-26-12(2)6-16(32)8-18(26)34/h5-10,29-34H,1-4H3

InChI key

VPEASJIRGSVXBF-UHFFFAOYSA-N

biological source

synthetic

agency

certified by the BSC

form

powder

technique(s)

microbe id | staining: suitable

solubility

1 M NaOH: 1 mg/mL

εmax

≥250% at 574-582 nm in NaOH at 0.01 M

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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Application

经认证可用于 Darrow′s 弹性纤维染色;乙肝表面抗原的紫罗兰素法。
Orcein is suitable for various applications, including:
  • Staining Elastic Fibers: Commonly used to stain elastic fibers and lamellae in tissue sections, facilitating the study of connective tissue architecture.
  • Whole Mount and Cryosection Staining: Effective for staining whole mounts and cryosections, allowing for detailed visualization of tissue morphology.
  • Quantification of Elastin: Utilized in quantifying elastin content in tissues, which is vital for understanding various pathological conditions.
  • Fluorescent Staining: Employed for fluorescent staining of elastic fibres in epoxy resin sections, enhancing the detection of specific tissue components.
  • Demonstration of Connective Tissue Elements: Used to visualize related connective tissue structures, including elaunin and oxytalan fibers.
  • Chromosome Staining: Applied in the staining of chromosomes in plants and human sex chromatin, aiding in genetic and cytogenetic studies.
  • Nuclear Staining: Serves as a nuclear stain for whole mount staining of embryos and assessing oocyte maturation, contributing to developmental biology research.
  • In-Situ Hybridization: Recommended for determining stages of meiosis in plant meiocytes, enhancing genetic research capabilities.
  • Hepatic Inclusions Demonstration: Useful in demonstrating hepatic inclusions, particularly hepatitis B antigen and copper-protein deposits, aiding in liver pathology studies.

General description

Orcein (Cl Natural Red 28) is a versatile dye known for its exceptional staining properties, particularly for elastic fibres and lamellae in histological samples. With an absorption maximum of 575 nm in an aqueous solution at pH 9.2 and an emission maximum of 585-590 nm, Orcein is widely used in histology and cytology for its ability to highlight connective tissue elements. The dye exhibits varied solubility depending on the batch and pH, ranging from practically insoluble to 2% soluble in water, and from 0.1% to 4% in ethanol.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Journal of clinical pathology, 61(4), 504-508 (2007-10-30)
To describe the histological features of the liver in patients with a Fontan circulation. Specimens from liver biopsies carried out as part of preoperative assessment prior to extracardiac cavopulmonary conversion of an older style Fontan were examined and scored semi-quantitatively

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