产品名称
地衣红, synthetic, certified by the BSC
SMILES string
N(c5c(cc(cc5C)O)O)C1=C(c2[c]([o][c]3c2C(=C(C(=O)C=3)Nc4c(cc(cc4C)O)O)C)=CC1=O)C
InChI
1S/C28H24N2O7/c1-11-5-15(31)7-17(33)25(11)29-27-13(3)23-21(9-19(27)35)37-22-10-20(36)28(14(4)24(22)23)30-26-12(2)6-16(32)8-18(26)34/h5-10,29-34H,1-4H3
InChI key
VPEASJIRGSVXBF-UHFFFAOYSA-N
biological source
synthetic
agency
certified by the BSC
form
powder
technique(s)
microbe id | staining: suitable
solubility
1 M NaOH: 1 mg/mL
εmax
≥250% at 574-582 nm in NaOH at 0.01 M
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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General description
Orcein (Cl Natural Red 28) is a versatile dye known for its exceptional staining properties, particularly for elastic fibres and lamellae in histological samples. With an absorption maximum of 575 nm in an aqueous solution at pH 9.2 and an emission maximum of 585-590 nm, Orcein is widely used in histology and cytology for its ability to highlight connective tissue elements. The dye exhibits varied solubility depending on the batch and pH, ranging from practically insoluble to 2% soluble in water, and from 0.1% to 4% in ethanol.
Application
经认证可用于 Darrow′s 弹性纤维染色；乙肝表面抗原的紫罗兰素法。
Orcein is suitable for various applications, including:
- Staining Elastic Fibers: Commonly used to stain elastic fibers and lamellae in tissue sections, facilitating the study of connective tissue architecture.
- Whole Mount and Cryosection Staining: Effective for staining whole mounts and cryosections, allowing for detailed visualization of tissue morphology.
- Quantification of Elastin: Utilized in quantifying elastin content in tissues, which is vital for understanding various pathological conditions.
- Fluorescent Staining: Employed for fluorescent staining of elastic fibres in epoxy resin sections, enhancing the detection of specific tissue components.
- Demonstration of Connective Tissue Elements: Used to visualize related connective tissue structures, including elaunin and oxytalan fibers.
- Chromosome Staining: Applied in the staining of chromosomes in plants and human sex chromatin, aiding in genetic and cytogenetic studies.
- Nuclear Staining: Serves as a nuclear stain for whole mount staining of embryos and assessing oocyte maturation, contributing to developmental biology research.
- In-Situ Hybridization: Recommended for determining stages of meiosis in plant meiocytes, enhancing genetic research capabilities.
- Hepatic Inclusions Demonstration: Useful in demonstrating hepatic inclusions, particularly hepatitis B antigen and copper-protein deposits, aiding in liver pathology studies.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Sharma AK and Sharma A
Chromosome Techniques: Theory and Practice (2014)
Kei Sakamoto et al.
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 41(8), 637-641 (2012-04-11)
A Rushton's hyaline body (HB) is a concretion occasionally found in odontogenic cysts. Unspecified substances produced by the lining epithelium or derived from blood components have been suggested as possible causes of HB formation, but the origin of HBs is
Juan Du et al.
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences, 38(3), 318-320 (2006-06-17)
To compare the advantages and disadvantages of three staining methods of HBV. Normal Liver tissue and HBV-infected, HCV-infected, or dually infected (HBV and HCV) liver tissues were selected for this study. Formalin-fixed, paraffin-embedded sections (4 microm) were prepared. Each of
Sergio Pimpinelli et al.
Cold Spring Harbor protocols, 2010(3), pdb-pdb (2010-03-03)
In this protocol, larval brains from Drosophila are incubated in vitro with colchicine, treated with hypotonic solution, fixed, and squashed in aceto-orcein. This procedure provides a large number of well-spread metaphase figures (200-400 per brain) that can be analyzed for
K Demirtaş et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 82(6), 319-323 (2007-12-22)
It is difficult to distinguish muscle, vessel, nerve, fascia, tendon and many other structures in fetal sections. The aim of the study reported here was to research the practicability of orcein-picroindigocarmine staining for distinguishing anatomic structures in histological sections containing
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