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O5003

Sigma-Aldrich

OPI 培养基添加剂

Hybri-Max, γ-irradiated, powder, suitable for hybridoma

别名:

OPI Media for cell culture

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

等级

Hybri-Max

质量水平

无菌性

γ-irradiated

形式

powder

浓度

18-40 mg/vial

技术

cell culture | hybridoma: suitable

杂质

endotoxin, tested

储存温度

−20°C

相关类别

应用

OPI培养基添加剂已用于转基因小鼠(XenoMouse)品系和BALB/c雌鼠来源的杂交瘤细胞系生长。

其他说明

配方含有:0.15g草酰乙酸,0.05g丙酮酸盐和0.0082g胰岛素。

重悬

10mL 无菌水可复原一小瓶本品。初次复原时请勿使用细胞培养基。每小瓶足以制备 1 升培养基。最终工作浓度:1mM 草酰乙酸盐、0.45mM 丙酮酸盐、0.2U/ml 胰岛素。

法律信息

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

象形图

CorrosionExclamation mark

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Skin Corr. 1 - Skin Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
Jun Yang et al.
Clinical biochemistry, 48(13-14), 843-848 (2015-05-31)
Neurogranin (NRGN) is a small neuronal protein that plays an important role in synaptic signaling by regulating calmodulin (CaM) availability. In this study, we developed an ELISA to measure NRGN quantitatively in serum samples from a cohort of acute traumatic
Gabriela A Canziani et al.
Analytical biochemistry, 325(2), 301-307 (2004-01-31)
Experimental and data analysis protocols were developed to screen antibodies from hybridoma culture supernatants using Biacore surface plasmon resonance biosensor platforms. The screening methods involved capturing antibodies from crude supernatants using Fc-specific antibody surfaces and monitoring antigen binding at a
Tzu-Ling Sung et al.
PLoS pathogens, 5(1), e1000263-e1000263 (2009-01-17)
Cyclin T1 is a regulatory subunit of a general RNA polymerase II elongation factor known as P-TEFb. Cyclin T1 is also required for Tat transactivation of HIV-1 LTR-directed gene expression. Translation of Cyclin T1 mRNA has been shown to be
Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring

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