质量水平
用途
kit sufficient for 5 preparations
环保替代产品特性
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
环保替代产品分类
, Aligned
储存温度
15-25°C
一般描述
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
GenElute HP Endotoxin-Free Plasmid Megaprep Kit 可以简单、快速、低成本的为从重组大肠杆菌培养物中分离不含内毒素的质粒DNA。在1.5小时内可从200 mL - 1.0L LB培养基或200 - 600 mL大肠杆菌TB培养基纯化出多达5 mg内毒素水平≤ 0.1 EU/μg高拷贝数质粒DNA。请注意，实际得率取决于所用的菌株、质粒和培养基。
该产品可为包含内毒素敏感细胞的限制性酶切、连接、测序、PCR、转化和转染等下游应用提供优质、不含内毒素的DNA。
该产品可为包含内毒素敏感细胞的限制性酶切、连接、测序、PCR、转化和转染等下游应用提供优质、不含内毒素的DNA。
应用
该产品获得高质量、不含内毒素的DNA，得到的DNA可进行诸多下游应用，如限制性酶切、连接、测序、PCR、转化和内毒素敏感细胞的转染等。
GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.
特点和优势
- 可纯化出多达5 mg内毒素水平≤0.1 EU/μg高拷贝数质粒DNA
- 从沉淀细胞到获得纯化的质粒，仅需90分钟
- 采用真空处理形式，无需进行乙醇沉淀
其他说明
更多信息，请见www.sigma-aldrich.com/genelutehp。
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
靶器官
Central nervous system, Lungs
储存分类代码
3 - Flammable liquids
闪点(°F)
77.0 °F - closed cup
闪点(°C)
25 °C - closed cup
法规信息
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