一般描述
GenElute HP 无内毒素质粒大量制备试剂盒可以简单、快速地从重组大肠杆菌培养物中分离不含内毒素的质粒DNA。该试剂盒采用了可快速澄清细菌裂解液的滤柱和可捕获质粒DNA的硅胶柱。配套的专利溶液可使质粒DNA结合到结合柱上，同时避免内毒素随之吸附。该技术使得用户可以维持一致的、低于 0.1 EU/μg 质粒DNA的内毒素水平。
制备所得的优质、不含内毒素DNA可直接供包括内毒素敏感细胞转染等最严格的应用使用。
制备所得的优质、不含内毒素DNA可直接供包括内毒素敏感细胞转染等最严格的应用使用。
应用
GenElute™ HP Endotoxin-Free Plasmid Maxiprep试剂盒已被用于分离猪肝和大肠杆菌感受态细胞中的质粒。
特点和优势
- 可纯化出多达1.2 mg内毒素水平≤0.1 EU/μg高拷贝数质粒DNA
- 从沉淀细胞到获得纯化的质粒，仅需35分钟
- 方便的真空形式
原理
通过离心分离法收集过夜的重组大肠杆菌培养液，然后通过改性SDS 碱裂解法进行处理。过滤澄清裂解液，然后加入针对无内毒素质粒制备而优化的结合溶液。随后，质粒捕获到硅胶膜上，同时避免内毒素随之吸附到膜上。通过两遍洗涤步骤，去除污染物。最后，在不含内毒素的水中洗脱结合所得的DNA。回收的质粒DNA主要以超螺旋形式存在。所含的基因组DNA和RNA均低于溴化丙啶染色琼脂糖凝胶电泳的检测水平。
其他说明
更多信息，请见www.sigma-aldrich.com/genelutehp。
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system
储存分类代码
3 - Flammable liquids
闪点(°F)
77.0 °F - closed cup
闪点(°C)
25 °C - closed cup
法规信息
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