一般描述
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比标准DNA提取方法使用苯酚和氯仿，该产品是一种更安全的化学产品。
应用
GenElute™ HP 质粒Midiprep试剂盒已用于分离和纯化质粒。
特点和优势
- 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
- 可纯化出多达350 μg高拷贝数质粒DNA
- 真空或离心形式可灵活选择
- 无需进行苯酚/氯仿萃取或乙醇沉淀步骤
- 所含的塑料成分更少，减少了废物量
其他说明
更多信息，请见www.sigma-aldrich.com/genelutehp。
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
靶器官
Central nervous system
储存分类代码
3 - Flammable liquids
法规信息
动植物源性产品
危险化学品
Vanessa E Gray et al.
G3 (Bethesda, Md.), 9(11), 3683-3689 (2019-09-29)
Despite the importance of Aβ aggregation in Alzheimer's disease etiology, our understanding of the sequence determinants of aggregation is sparse and largely derived from in vitro studies. For example, in vitro proline and alanine scanning mutagenesis of Aβ40 proposed core
Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
Steinhart Z
Nature Medicine, 23(1), 60-68 (2017)
The distal upstream promoter in Ly49 genes, Pro1, is active in mature NK cells and T cells, does not require TATA boxes, and displays enhancer activity.
Gays F
Journal of Immunology, 194(12), 6068-6081 (2015)
Stephanie P Cartwright et al.
BMC research notes, 10(1), 340-340 (2017-07-29)
Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV
Melissa A Chiasson et al.
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Vitamin K epoxide reductase (VKOR) drives the vitamin K cycle, activating vitamin K-dependent blood clotting factors. VKOR is also the target of the widely used anticoagulant drug, warfarin. Despite VKOR's pivotal role in coagulation, its structure and active site remain
实验方案
使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。
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DNA和RNA提取是分子生物学中的一种基本方法。高质量、高纯度的核酸在一系列研究和临床应用中发挥着重要的作用。因此，核酸纯化是分子生物学和基因组工作流程中的第一步。
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
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