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Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

For important updates on recent policy changes, please click here for more details.

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安全信息

NA0200

Sigma-Aldrich

GenElute™ HP质粒中量制备试剂盒

greener alternative

sufficient for 25 purifications

别名:

GenElute™ HP质粒中量制备试剂盒, Gen Elute

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1 KIT
CN¥4,034.43

CN¥4,034.43

国内现货，预计发货时间April 17, 2025详情

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1 KIT
CN¥4,034.43

About This Item

UNSPSC代码:
41105501
NACRES:
NA.52

CN¥4,034.43

国内现货，预计发货时间April 17, 2025详情

用途

sufficient for 25 purifications

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

DNA purification: suitable

环保替代产品分类

, Aligned

储存温度

15-25°C

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1 of 4

此商品
NA0300NA0310PLN70
GenElute™ HP Plasmid Midiprep Kit sufficient for 25 purifications

NA0200

GenElute™ HP Plasmid Midiprep Kit

GenElute™ HP Plasmid Maxiprep Kit sufficient for 10 purifications

NA0300

GenElute™ HP Plasmid Maxiprep Kit

GenElute™ HP Plasmid Maxiprep Kit sufficient for 25 purifications

NA0310

GenElute™ HP Plasmid Maxiprep Kit

GenElute™ Plasmid Miniprep Kit sufficient for 70 purifications

PLN70

GenElute™ Plasmid Miniprep Kit

usage

sufficient for 25 purifications

usage

sufficient for 10 purifications

usage

sufficient for 25 purifications

usage

sufficient for 70 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

NA0200S

NA0300S, NA0310

NA0300, NA0300S

PLN10, PLN350

greener alternative category

, Aligned

greener alternative category

Aligned,

greener alternative category

Aligned,

greener alternative category

, Aligned

一般描述

GenElute HP质粒中量制备试剂盒可以超快速、高效的从重组大肠杆菌培养物大批量制备质粒。该试剂盒结合了硅胶膜技术和离心或真空形式的便利性。该试剂盒还附带专用的滤器卡夹，从而替代了碱裂解之后的离心分离步骤。裂解之后，DNA结合到硅胶膜上，并通过一次简单的洗涤步骤去除了污染物。随后在水或TE缓冲液中，通过离心处理洗脱DNA。

纯化所得的质粒DNA适于包括限制性酶切和测序在内的一系列分子生物学应用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比标准DNA提取方法使用苯酚和氯仿，该产品是一种更安全的化学产品。

应用

GenElute™ HP 质粒Midiprep试剂盒已用于分离和纯化质粒。[1][2]

特点和优势

  • 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
  • 可纯化出多达350 μg高拷贝数质粒DNA
  • 真空或离心形式可灵活选择
  • 无需进行苯酚/氯仿萃取或乙醇沉淀步骤
  • 所含的塑料成分更少，减少了废物量

其他说明

更多信息，请见www.sigma-aldrich.com/genelutehp。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

靶器官

Central nervous system

储存分类代码

3 - Flammable liquids

法规信息

动植物源性产品
危险化学品

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000385570
    0000333635
    0000385570
    0000333635
    SLCP2413

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    Vanessa E Gray et al.
    G3 (Bethesda, Md.), 9(11), 3683-3689 (2019-09-29)
    Despite the importance of Aβ aggregation in Alzheimer's disease etiology, our understanding of the sequence determinants of aggregation is sparse and largely derived from in vitro studies. For example, in vitro proline and alanine scanning mutagenesis of Aβ40 proposed core
    Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
    Steinhart Z
    Nature Medicine, 23(1), 60-68 (2017)
    Stephanie P Cartwright et al.
    BMC research notes, 10(1), 340-340 (2017-07-29)
    Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV
    The distal upstream promoter in Ly49 genes, Pro1, is active in mature NK cells and T cells, does not require TATA boxes, and displays enhancer activity.
    Gays F
    Journal of Immunology, 194(12), 6068-6081 (2015)
    Theresa B Loveless et al.
    Nature chemical biology, 17(6), 739-747 (2021-03-24)
    Studying cellular and developmental processes in complex multicellular organisms can require the non-destructive observation of thousands to billions of cells deep within an animal. DNA recorders address the staggering difficulty of this task by converting transient cellular experiences into mutations
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    实验方案

    利用 CRISPR-Cas9 基因组编辑技术创建转基因小鼠

    使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。

    相关内容

    DNA & RNA Purification

    Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

    DNA纯化_RNA提取_核酸纯化-默克生命科学

    DNA和RNA提取是分子生物学中的一种基本方法。高质量、高纯度的核酸在一系列研究和临床应用中发挥着重要的作用。因此，核酸纯化是分子生物学和基因组工作流程中的第一步。

    我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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