推荐产品
用途
sufficient for 70 preparations
质量水平
环保替代产品特性
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技术
DNA extraction: suitable
环保替代产品分类
储存温度
15-25°C
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一般描述
通过离心收集过夜培养的重组 大肠杆菌，并进行改良的碱-SDS裂解步骤，然后在高盐存在下将质粒DNA吸附至二氧化硅上。然后，通过真空或旋转洗涤步骤去除污染物。最后，用水或Tris-EDTA缓冲液对结合的质粒DNA进行洗脱。
GenElute™ HP质粒试剂盒为从 大肠杆菌 培养物中进行质粒制备提供了一种超快速有效的解决方案。该试剂盒结合了基于二氧化硅的膜技术和旋转或真空形式的便利性，可在不到30分钟的时间内从1-5 mL的 大肠杆菌 培养物中最高可达获得25 μg的高拷贝质粒DNA。注意，实际的产量和最佳培养量取决于质粒和培养基。
GenElute™ HP质粒试剂盒为从 大肠杆菌 培养物中进行质粒制备提供了一种超快速有效的解决方案。该试剂盒结合了基于二氧化硅的膜技术和旋转或真空形式的便利性，可在不到30分钟的时间内从1-5 mL的 大肠杆菌 培养物中最高可达获得25 μg的高拷贝质粒DNA。注意，实际的产量和最佳培养量取决于质粒和培养基。
我们致力于为您带来更加绿色的替代产品，这些产品遵守一项或多项绿色化学12项原则。相比标准DNA提取方法使用苯酚和氯仿，该产品是一种更安全的化学产品。
应用
分离所得的质粒DNA可直接用于限制性酶切、克隆、PCR、转录和测序等下游应用。
特点和优势
- 可在30分钟内获得纯化的质粒DNA
- 可纯化出多达25 μg高拷贝数质粒DNA
- 真空或离心形式可灵活选择
- 无需进行苯酚/氯仿萃取或乙醇沉淀步骤
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system
WGK
WGK 2
闪点(°F)
77.0 °F
闪点(°C)
25 °C
法规信息
动植物源性产品
危险化学品
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