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重组
expressed in E. coli
表单
solution
enzyme activity
≥250 units/mg protein
分子量
230 kDa
运输
dry ice
储存温度
−70°C
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一般描述
Polynucleotide phosphorylase (PNPase) is required for cell viability and mRNA turnover in Escherichia coli.
应用
Polynucleotide phosphorylase (PNP) has been used in a study to show that spontaneous mutations resulting from replication errors are reduced in a PNP-deficient strain. It has also been used in a study to show that the absence of PNPase makes E. coli cells sensitive to UV, which suggests PNP has a role in survival of UV damage.
生化/生理作用
Polynucleotide phosphorylase from Escherichia coli functions as a exonuclease as well as a poly(A) polymerase and can add C and U nucleotides to poly(A) tails.
单位定义
One unit will polymerize 1.0 μmole of ADP releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C.
外形
Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 200 mM KCl, 21.4% (w/v) Glycerol
法规信息
新产品
Involvement of pnp in survival of UV radiation in Escherichia coli K-12
Rath, D., et al.
Annual Review of Microbiology, 128, 1196-1205 (2012)
W P Donovan et al.
Proceedings of the National Academy of Sciences of the United States of America, 83(1), 120-124 (1986-01-01)
The isolation of a temperature-sensitive allele of RNase II (rnb) by in vitro mutagenesis has permitted the demonstration that RNase II and polynucleotide phosphorylase (PNPase) are required for cell viability and mRNA turnover in Escherichia coli. Double-mutant strains carrying the
A J Carpousis
Biochemical Society transactions, 30(2), 150-155 (2002-05-31)
mRNA instability is an intrinsic property that permits timely changes in gene expression by limiting the lifetime of a transcript. The RNase e of Escherichia coli is a single-strand-specific endo-nuclease involved in the processing of rRNA and the degradation of
H Soreq et al.
The Journal of biological chemistry, 252(19), 6885-6888 (1977-10-10)
A simple procedure for purifying polynucleotide phosphorylase from Escherichia coli cells by means of affinity chromatography on an RNA-Sepharose column is described. The purified enzyme preparation has a specific activity 3500-fold that of the crude extract and is essentially homogeneous
Purification and properties of polynucleotide phosphorylase from Escherichia coli.
Y Kimhi et al.
The Journal of biological chemistry, 243(2), 231-240 (1968-01-25)
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