推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~55 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells
western blot: 1-2 μg/mL using NIH-3T3 lysate
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... NONO(4841)
mouse ... Nono(53610)
rat ... Nono(317259)
一般描述
Non-POU Domain-Containing Octamer-Binding Protein (NONO) is ubiquitously expressed and is composed of two tandem RNP-type RNA-recognition motifs and a putative helix-turn-helix domain followed by a highly charged region. It is enriched in paraspeckles, a nucleoplasmic compartment, and relocalizes to cap structures at the nucleolar periphery when transcription is inhibited.
Non-POU domain containing octamer binding protein (NONO) is part of the Drosophila behavior/human splicing (DBHS) protein family. It has a molecular weight of 54kDa and the gene encoding it is localized on human chromosome Xq13.1.
应用
Anti-NONO (N-terminal) antibody produced in rabbit has been used in immunoprecipitation, immunoblotting. It may be used for immunofluorescence.
生化/生理作用
Non-POU Domain-Containing Octamer-Binding Protein (NONO) is a nuclear protein implicated in numerous processes including transcription, pre-mRNA processing, nuclear retention of edited RNA, and DNA relaxation. NONO is phosphorylated on multiple sites during mitosis and is a target of the peptidylprolyl isomerase (Pin1).
Non-POU domain containing octamer binding protein (NONO) modulates transcription. Loss-of-function of the protein has been linked to human intellectual disability. The protein also has a role in synaptic transcription and regulation of the circadian clock. NONO also has a function in DNA damage response.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs.
Wang J, et al.
The Journal of Clinical Investigation, 127(3), 987-1004 (2017)
IGFBP-3 interacts with NONO and SFPQ in PARP-dependent DNA damage repair in triple-negative breast cancer.
de Silva HC, et al.
Cellular and Molecular Life Sciences, 76(10), 2015-2030 (2019)
Promoter-dependent translation controlled by p54nrb and hnRNPM during myoblast differentiation.
Ainaoui N, et al.
Testing, 10(9), e0136466-e0136466 (2015)
The multifunctional nuclear protein p54nrb is multiphosphorylated in mitosis and interacts with the mitotic regulator Pin1.
Proteau A, et al.
Journal of molecular biology, 346(4), 1163-1172 (2005)
Prefrontal cortex shotgun proteome analysis reveals
altered calcium homeostasis and immune system
imbalance in schizophrenia
altered calcium homeostasis and immune system
imbalance in schizophrenia
Daniel Martins-de-Souza
European Archives of Psychiatry and Clinical Neuroscience (2009)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门