N8664
Anti-NONO (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-NMT55, Anti-NRB54, Anti-Non-pou domain-containing octamer-binding protein, Anti-Nuclear RNA-binding protein, 54-KD, Anti-p54nrb
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所有图片(3)
About This Item
UNSPSC代码:
12352203
NACRES:
NA.41
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~55 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells
western blot: 1-2 μg/mL using NIH-3T3 cell lysates
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... NONO(4841)
一般描述
Non-POU domain containing octamer binding (NONO) is ubiquitously expressed and comprises of two tandem RNP-type RNA- recognition motifs and a putative helix-turn-helix domain followed by a highly charged region. It is enriched in paraspeckles, a nucleoplasmic compartment and relocalizes to cap structures at the nucleolar periphery when transcription is inhibited.
Non-POU domain containing octamer binding protein (NONO) is part of the Drosophila behavior/human splicing (DBHS) protein family. It has a molecular weight of 54kDa and the gene encoding it is localized on human chromosome Xq13.1.
应用
Anti-NONO (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Anti-NONO (N-terminal) antibody produced in rabbit has been used in immunoprecipitation.
生化/生理作用
NONO (also termed as p54nrb) is a nuclear protein implicated in numerous processes including transcription, pre-mRNA processing, nuclear retention of edited RNA and DNA relaxation. NONO was suspected to be involved in pre-mRNA splicing. Later it was found that NONO and PSF mediate different functions depending on their intracellular location.
Non-POU domain containing octamer binding protein (NONO) modulates transcription. Loss-of-function of the protein has been linked to human intellectual disability. The protein also has a role in synaptic transcription and regulation of the circadian clock. NONO also has a function in DNA damage response.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Prefrontal cortex shotgun proteome analysis reveals
altered calcium homeostasis and immune system
imbalance in schizophrenia
altered calcium homeostasis and immune system
imbalance in schizophrenia
Daniel Martins-de-Souza
European Journal of Anaesthesiology. Supplement (2009)
A crystallographic study of human NONO (p54nrb): overcoming pathological problems with purification, data collection and noncrystallographic symmetry
Knott GJ, et al.
Acta crystallographica. Section D, Structural biology, 761-761 (2016)
Paraspeckles: a novel nuclear domain.
Fox AH, et al.
Current Biology, 12(1), 13-25 (2002)
Characterization of DNA binding and pairing activities associated with the native SFPQ?NONO DNA repair protein complex
Durga Udayakumar
Biochemical and Biophysical Research Communications (2015)
NONO regulates the intra-S-phase checkpoint in response to UV radiation.
Alfano L
Oncogene (2016)
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