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安全信息

N8630

Sigma-Aldrich

核酸酶P1 来源于桔青霉菌

lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)

别名:

3′-磷酸水解酶, 核酸酶 5′-核苷酸水解酶, 内切核酸酶P1

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About This Item

CAS号:
54576-84-0
EC 号:
3.1.30.1 (BRENDA, IUBMB)
MDL编号:
MFCD00131010
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

Penicillium citrinum

质量水平

200

表单

lyophilized powder

比活

≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)

secondary activity

≥1,000 units/mg protein 3′-nucleotidase

分子量

42-50 kDa

包装

vial of ≥250 units (using RNA substrate)

技术

DNA extraction: suitable
DNA purification: suitable

适用性

suitable for molecular biology

应用

cell analysis

储存温度

2-8°C

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一般描述

核酸酶P1是分子生物学领域最为人熟知的单链特异性核酸酶之一；核酸酶P1是单链特异性内切酶（ssDNA或ssRNA内切酶）。核酸酶P1还可切割双链核酸中的单链区域。
柑桔青霉来源核酸酶P1是一种锌依赖性内切核酸酶，在存在低浓度尿素的情况下表现出更强的活性。 核酸酶P1的选择性活性现已可用于核酸结构研究应用。

应用

  • 单链DNA或RNA经核酸酶P1切割为5'单核苷酸
  • 核酸酶P1可用于支持DNA损伤和修饰研究
  • 借助核酸酶P1，可完整核酸碱基相关的组分和结构分析
  • 核酸酶P1一度用于从酵母RNA工业生产5′-单核苷酸。
  • 通过核酸酶P1，可在蛋白质纯化过程中去除核酸
  • 核酸酶P1是涉及t-RNA依赖性氨基酸生物合成和t-RNA依赖性转酰胺基的研究方法开发所需的关键试剂
  • 柑桔青霉来源核酸酶P1已用于一项使用硫酸铵或聚乙二醇4000作为沉淀剂从而评估晶体结构的研究。
  • 核酸酶P1还用于一项研究方法调查，可从5′或3′端基标记的RNA，直接对20-25个核苷酸进行序列分析。
  • 核酸酶P1用于提高基于32P标记进行DNA加合物检测方法的灵敏度。
该酶具有约70°C的最适温度，但低于60°C的温度对于更长时间的孵育更为合适。在pH 5 - 8范围内稳定。
该酶具有约70 °C的最适温度，但低于60 °C的温度对于更长时间的孵育更为合适。它可稳定在pH 5 - 8范围内。

生化/生理作用

催化对单链DNA和RNA的非特异性核酸酶内切以产生核苷5′-磷酸和5′-磷酸寡核苷酸。它不会对双链的核酸具有明显的降解，特别是在pH6.0并有超过400 mM氯化钠存在的情况下。

特点和优势

我们的高活性核酸酶P1已通过3′- 5′ - 磷酸二酯酶活性和3′- 核苷酸酶活性测试，且是市场上活性最高的核酸酶P1

物理属性

由270个氨基酸残基组成的一种锌依赖性糖蛋白。分子量：42-50 kDa。

单位定义

3′-5′-磷酸二酯酶：一单位的酶在37°C、pH 5.3条件下每分钟可从RNA释放1.0 μ摩尔的酸可溶性核苷酸。
3′-核酸酶：一单位的酶在37°C、pH 7.2条件下每分钟可将1.0 μ摩尔的正磷酸从3′-AMP中水解。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000399439
0000387196
0000281586
0000360402
0000340475

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Product N8630 (Nuclease P1 from Penicillium citrinum is a lyophilized powder. How should I store solutions of this enzyme?

    A solution of Nuclease P1 that has been reconstituted in 20 mM sodium acetate buffer (pH 5.3), 5 mM ZnCl2, and 50 mM sodium chloride can be stored for the short term at 2-8 °C for 2-3 weeks. For longer term storage, at -20 °C, one suggestion is to add 40-50% glycerol after reconstitution in the aqueous buffer. Such solutions (with glycerol added) may be stored for 3-6 months at -20 °C.

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    Ask a Scientist here.

M V Reddy et al.
Carcinogenesis, 7(9), 1543-1551 (1986-09-01)
Exceedingly sensitive procedures are required to detect the presence of covalent DNA adducts in humans exposed to environmental genotoxicants because of low levels of such derivatives (1 adduct in 10(8)-10(10) DNA nucleotides). A 32P-postlabeling assay for detection and quantitation of
Fahimeh Salehi et al.
Scientific reports, 8(1), 13902-13902 (2018-09-19)
DNA targeting anticancer agents have been very successful in clinic, especially, when used in combinatorial therapy. But unfortunately, they often exhibit high levels of toxicity towards normal cells. Hence, much effort has been put into finding agents with more selectivity
Enhancement of nuclease P1 activity in low concentration of denaturants
Gangadhara, K. and P. Gangadhara
Enzyme and Microbial Technology, 43, 7-7 (2008)
K Shanmugha Rajan et al.
Nucleic acids research, 47(14), 7633-7647 (2019-05-31)
The parasite Trypanosoma brucei, the causative agent of sleeping sickness, cycles between an insect and a mammalian host. Here, we investigated the presence of pseudouridines (Ψs) on the spliceosomal small nuclear RNAs (snRNAs), which may enable growth at the very
Xiaohuan Jin et al.
Nucleic acids research, 47(2), 883-898 (2018-12-07)
Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana.
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