N8010
4-Nitrophenyl 4-guanidinobenzoate hydrochloride
protease substrate, ≥95.0% (TLC), powder
别名:
4-Guanidinobenzoic acid 4-nitrophenylester hydrochloride, NPGB, pNPGB
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关于此项目
经验公式（希尔记法）:
C14H12N4O4 · HCl
化学文摘社编号:
分子量:
336.73
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
242-831-3
MDL number:
Beilstein/REAXYS Number:
8024554
产品名称
4-Nitrophenyl 4-guanidinobenzoate hydrochloride, protease inhibitor and substrate
InChI key
PKSBDZOBYIKNGY-UHFFFAOYSA-N
InChI
1S/C14H12N4O4.ClH/c15-14(16)17-10-3-1-9(2-4-10)13(19)22-12-7-5-11(6-8-12)18(20)21;/h1-8H,(H4,15,16,17);1H
SMILES string
Cl[H].NC(=N)Nc1ccc(cc1)C(=O)Oc2ccc(cc2)[N+]([O-])=O
assay
≥95.0% (TLC)
form
powder
solubility
formic acid: soluble 49.00-51.00 mg/mL
storage temp.
−20°C
Quality Level
General description
4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) is a trypsin substrate. It is more water-soluble than 4-methylumbelliferyl 4-guanidinobenzoate and is preferred in spectrophotometric assay.
Application
4-Nitrophenyl 4-guanidinobenzoate hydrochloride has been used:
- as a substrate for trypsin for active site titration experiments
- for pre-treating of mosquito eggs in the interplasmid transposition assay
- as a component of isotonic buffer to moisten filter paper for mosquito embryo collection
Biochem/physiol Actions
4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Mélia Magnen et al.
Biological chemistry, 399(9), 1053-1064 (2018-06-09)
Every year, influenza A virus (IAV) affects and kills many people worldwide. The viral hemagglutinin (HA) is a critical actor in influenza virus infectivity which needs to be cleaved by host serine proteases to exert its activity. KLK5 has been
M Llanos et al.
Journal of reproduction and fertility, 97(1), 173-178 (1993-01-01)
In this study we evaluated the effect of several trypsin inhibitors (p-aminobenzamidine: pAB; N-alpha-p-tosyl-L-lysine-chloromethyl-ketone: TLCK and p-nitrophenyl-p'-guanidino-benzoate: NPGM) on sperm binding and penetration of the human zona pellucida. Motile spermatozoa, selected by a two-step Percoll gradient, were incubated at 1
F Catteruccia et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(5), 2157-2162 (2000-02-19)
The ability of the Minos transposable element to function as a transformation vector in anopheline mosquitoes was assessed. Two recently established Anopheles gambiae cell lines were stably transformed by using marked Minos transposons in the presence of a helper plasmid
J E Dravland et al.
The Journal of experimental zoology, 232(1), 117-128 (1984-10-01)
Previous studies from this laboratory have suggested a role for sperm trypsinlike activity in the membrane events of the hamster sperm acrosome reaction, but these conclusions have been disputed by studies done with guinea pig, mouse, and ram sperm. This
Studies on the Molecular Mechanism of Mersalyl and 4-Aminophenylmercuric Acetate Re-activation of Trypsin-Thiol Complexes
STEVEN FS and GRIFFIN MM
European Journal of Biochemistry, 109(2), 567-573 (1980)
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