一般描述
神经氨酸酶是糖苷水解酶，催化末端唾液酸残基的水解。最知名的莫过于促进流感病毒释放的病毒近酰胺酶。
应用
来自霍乱弧菌的神经氨酸苷酶已在一项研究中使用，以评估其在易感细胞结合和摄取霍乱毒素中的作用。它还已在另一项研究中使用，以研究钠底物的制备及其在神经氨酸酶荧光测定中的应用。
神经氨酸酶被用作糖缀合物分布和底物特异性研究的细胞表面探针。
生化/生理作用
霍乱弧菌神经氨酸酶已被证明通过结合霍乱毒素并帮助其被细胞摄取来促进霍乱毒素感染。
质量
不含防腐剂。
单位定义
除非另有说明，否则在37 °条件下，当pH为5.0时，使用NAN-乳糖或牛颌下粘蛋白，一个单位每分钟将释放1.0 μ摩尔的N-乙酰神经氨酸。价格基于将NAN-乳糖用作底物的单位。
外形
水溶液，pH 5.5，含0.15 M NaCl和4 mM CaCl2。
制备说明
色谱纯化产品。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Paola Rota et al.
Organic & biomolecular chemistry, 10(14), 2885-2894 (2012-03-08)
A simple protocol for the synthesis of N-perfluoroacylated and N-acylated glycals of neuraminic acid, with a secondary cyclic amine (morpholine or piperidine) at the 4α position, has been set-up, starting from peracetylated N-acetylneuraminic acid methyl ester that undergoes, sequentially to
Jai Woong Seo et al.
Nature communications, 11(1), 2102-2102 (2020-05-02)
Adeno-associated viruses (AAVs) are typically single-stranded deoxyribonucleic acid (ssDNA) encapsulated within 25-nm protein capsids. Recently, tissue-specific AAV capsids (e.g. PHP.eB) have been shown to enhance brain delivery in rodents via the LY6A receptor on brain endothelial cells. Here, we create
P C Reading et al.
Journal of virology, 74(11), 5190-5197 (2000-05-09)
Influenza viruses A/PR/8/34 (PR8; H1N1), A/Aichi/68 X-31 (HKx31; H3N2), and A/Beijing/89 X-109 (BJx109; H3N2) show marked differences in their ability to infect murine macrophages, including resident alveolar and peritoneal macrophages as well as the macrophage-derived cell line J774. The hierarchy
Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate.
M Potier et al.
Analytical biochemistry, 94(2), 287-296 (1979-04-15)
L Patrick Havlik et al.
Journal of virology, 95(19), e0058721-e0058721 (2021-07-08)
Adeno-associated viruses utilize different glycans and the AAV receptor (AAVR) for cellular attachment and entry. Directed evolution has yielded new AAV variants; however, structure-function correlates underlying their improved transduction are generally overlooked. Here, we report that infectious cycling of structurally
商品
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
实验方案
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
在测定神经氨酸酶活性时，使用了一种以N-乙酰新胺酸为酶底物的比色法进行测定。神经氨酸酶是一种可促进流感病毒从感染细胞中释放的水解酶。
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