N7885
神经氨酸酶 来源于霍乱弧菌
Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)
别名:
受体破坏酶, 唾液酸酶, 神经氨酸苷酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-624-6
MDL number:
Specific activity:
1-5 units/mg protein (Lowry, using NAN-lactose)
Quality Level
type
Type III
form
buffered aqueous solution
specific activity
1-5 units/mg protein (Lowry, using NAN-lactose)
mol wt
83 kDa
application(s)
diagnostic assay manufacturing
foreign activity
Protease and NAN-aldolase, present
storage temp.
2-8°C
General description
神经氨酸酶是糖苷水解酶，催化末端唾液酸残基的水解。最知名的莫过于促进流感病毒释放的病毒近酰胺酶。
Application
来自霍乱弧菌的神经氨酸苷酶已在一项研究中使用，以评估其在易感细胞结合和摄取霍乱毒素中的作用。它还已在另一项研究中使用，以研究钠底物的制备及其在神经氨酸酶荧光测定中的应用。
神经氨酸酶被用作糖缀合物分布和底物特异性研究的细胞表面探针。
Biochem/physiol Actions
霍乱弧菌神经氨酸酶已被证明通过结合霍乱毒素并帮助其被细胞摄取来促进霍乱毒素感染。
Physical form
水溶液，pH 5.5，含0.15 M NaCl和4 mM CaCl2。
Preparation Note
色谱纯化产品。
Analysis Note
不含防腐剂。
Other Notes
除非另有说明，否则在37 °条件下，当pH为5.0时，使用NAN-乳糖或牛颌下粘蛋白，一个单位每分钟将释放1.0 μ摩尔的N-乙酰神经氨酸。价格基于将NAN-乳糖用作底物的单位。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
高风险级别生物产品--人源产品
此项目有
实验方案
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
在测定神经氨酸酶活性时，使用了一种以N-乙酰新胺酸为酶底物的比色法进行测定。神经氨酸酶是一种可促进流感病毒从感染细胞中释放的水解酶。
商品
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
相关内容
Instructions
P C Reading et al.
Journal of virology, 74(11), 5190-5197 (2000-05-09)
Influenza viruses A/PR/8/34 (PR8; H1N1), A/Aichi/68 X-31 (HKx31; H3N2), and A/Beijing/89 X-109 (BJx109; H3N2) show marked differences in their ability to infect murine macrophages, including resident alveolar and peritoneal macrophages as well as the macrophage-derived cell line J774. The hierarchy
Jai Woong Seo et al.
Nature communications, 11(1), 2102-2102 (2020-05-02)
Adeno-associated viruses (AAVs) are typically single-stranded deoxyribonucleic acid (ssDNA) encapsulated within 25-nm protein capsids. Recently, tissue-specific AAV capsids (e.g. PHP.eB) have been shown to enhance brain delivery in rodents via the LY6A receptor on brain endothelial cells. Here, we create
Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate.
M Potier et al.
Analytical biochemistry, 94(2), 287-296 (1979-04-15)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N7885-1UN | 04061834118282 |
| N7885-2UN | 04061834118299 |