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Merck
CN

N5759

Sigma-Aldrich

4-硝基苯基 β- D -纤维二糖

chromogenic, ≥98% (TLC), powder

别名:

p -硝基苯基 β- D -纤维二糖

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About This Item

经验公式(希尔记法):
C18H25NO13
CAS号:
分子量:
463.39
Beilstein:
100234
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.32

产品名称

4-硝基苯基 β- D -纤维二糖, ≥98% (TLC)

质量水平

方案

≥98% (TLC)

表单

powder

溶解性

water: 49.00-51.00 mg/mL

储存温度

2-8°C

SMILES字符串

OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O[C@@H]2CO)Oc3ccc(cc3)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9-,10-,11-,12+,13-,14-,15-,16-,17-,18+/m1/s1

InChI key

IAYJZWFYUSNIPN-KFRZSCGFSA-N

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应用

4-硝基苯基β-D-吡喃木糖苷已用作底物,以测定β-D-纤维二糖苷酶或纤维二糖水解酶(胞外纤维素酶)在微生物群落中的活性。它还可用作底物来研究内切葡聚糖酶J30的活性。

生化/生理作用

4-硝基苯基 β-D-吡喃木糖苷是一种纤维三糖类似物,和常用于检测纤维素酶活性的显色底物。外切葡聚糖酶、内切葡聚糖酶和β -葡糖苷酶可水解4-硝基苯基 β-D-吡喃木糖苷,以形成p-硝基酚(PNP)。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

监管及禁止进口产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Keum Shin et al.
Journal of microbiology and biotechnology, 20(12), 1681-1688 (2011-01-05)
A screening for cellobiohydrolase (CBH) activity was performed and Fomitopsis pinicola KMJ812 was selected for further characterization as it produced a high level of CBH activity. An extracellular CBH was purified to homogeneity by sequential chromatography of F. pinicola culture
Effects of microplastic fibers on soil aggregation and enzyme activities are organic matter dependent
Liang Y, et al.
Frontiers in environmental science., 97 null
M Fukumura et al.
Bioscience, biotechnology, and biochemistry, 59(1), 47-50 (1995-01-01)
The thermal denaturation process of Clostridium stercorarium F-9 xylanase (XynB) was studied by monitoring remaining activity and recovered activity of the enzyme. At pH 5.5, aggregation occurred rapidly after the thermal denaturation initiated. The aggregated protein could be dissolved in
R M Gardner et al.
Journal of bacteriology, 169(10), 4581-4588 (1987-10-01)
An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter
S J Han et al.
The Journal of biological chemistry, 270(43), 26012-26019 (1995-10-27)
Bacillus sp. D04 secreted a bifunctional cellulase that had a molecular weight of 35,000. This cellulase degraded Cm-cellulose, cellotetraose, cellopentaose, p-nitrophenyl-beta-D-cellobioside, and avicel PH101. Based on the high performance liquid chromatography analysis of the degradation products, this cellulase randomly cleaved

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