N5415
神经生长因子-β 人
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
别名:
β-NGF, BNGF, NGF-β
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UNSPSC Code:
12352202
NACRES:
NA.77
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
protein calculated mol wt 13.5 kDa (Monomer)
Impurities:
≤1 EU/μg Endotoxin (β-NGF)
产品名称
神经生长因子-β 人, recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤16 ng/mL ED50/EC50
mol wt
protein calculated mol wt 13.5 kDa (Monomer)
packaging
pkg of 10 μg, pkg of 100 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg Endotoxin (β-NGF)
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... NGF(4803)
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General description
NGF-β基因定位于人类染色体1p13.2。
重组人β-NGF在人293细胞中以120个氨基酸的同型二聚体（非二硫键）表达，计算分子量为13.5 kDa（单体）。神经生长因子β（β-NGF）是一种在结构上与BDNF、NT-3和NT-4相关的神经营养因子。天然β-NGF的产生发生在上皮细胞和脑下垂体中。
Biochem/physiol Actions
NGF-β基因的突变与疼痛感的丧失有关。
神经生长因子β（β-NGF）是一种有效的神经营养因子，通过其受体β-NGFR发出信号，在感觉和交感神经系统的发育和保存中起着至关重要的作用。β-NGF促进基底前脑胆碱能神经元的存活和胆碱能表型，并在体外刺激胚胎三叉神经运动神经元的神经突向外生长。
Physical form
该产品从PBS中冻干获得。
Preparation Note
将产品储存在-20°C下。产品在供应时可保持至少2年的活性。初始解冻后，建议将蛋白以工作等分试样储存在-20 °C下。
打开前短暂离心小瓶。 建议在含有0.1%无内毒素重组人血清白蛋白的无菌DDW中复溶蛋白质，使其最终浓度为100 μg/mL。
Analysis Note
通过人TF-1细胞（人红白血病指示细胞系）增殖的剂量依赖性刺激测定比活性。
Disclaimer
产品在供应时可保持至少2年的活性。 初始解冻后，建议将蛋白以工作等分试样储存在-20℃下。
除非我们的目录中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
法规信息
监管及禁止进口产品
此项目有
A Rosenthal et al.
Handbook of experimental pharmacology, 220, 497-512 (2014-03-29)
The neurotrophin family is comprised of the structurally related secreted proteins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophine-4 (NT-4). They bind and activate the tyrosine kinase receptors Trk A, B, and C in a ligand-specific
Tissue localization of nerve growth factor and nerve growth factor receptors.
M Bothwell
Current topics in microbiology and immunology, 165, 55-70 (1991-01-01)
S Soinila et al.
Neuroscience research, 6(2), 123-130 (1988-12-01)
Nerve growth factor (NGF)-like immunoreactivity was demonstrated in the rat pituitary intermediate lobe by indirect immunofluorescence method using antisera specific to beta-NGF isolated from adult male mouse submaxillary salivary gland. Co-culture of frozen or fresh intermediate lobes with newborn rat
Katherine M Nielsen et al.
Molecular and cellular neurosciences, 25(4), 629-641 (2004-04-15)
Developing neurons encounter a panoply of extracellular signals as they differentiate. A major goal is to identify these extrinsic cues and define the mechanisms by which neurons simultaneously integrate stimulation by multiple factors yet initiate one specific biological response. Factors
T Lahtinen et al.
Neuroscience, 30(1), 165-170 (1989-01-01)
Explants of anterior, intermediate and posterior pituitary lobes of rats at various ages evoked an intense nerve fibre outgrowth when co-cultured with neonatal sympathetic superior cervical ganglia in a collagen gel medium. Freezing and thawing of pituitary lobes, prior to
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