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Merck
CN

N5386

Sigma-Aldrich

微球菌核酸酶 来源于金黄色葡萄球菌

100-300 units/mg protein

别名:

MNase, 微球菌核酸内切酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

Staphylococcus aureus

质量水平

表单

powder

比活

100-300 units/mg protein

组成

Protein, ≥40% E1%/280 (Balance primarily sodium acetate)

技术

DNA extraction: suitable
DNA purification: suitable

适用性

suitable for molecular biology

应用

cell analysis

储存温度

−20°C

基因信息

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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应用

微球菌核酸酶可用于消化染色质以鉴定核小体的位置。
来源于金黄色葡萄球菌的微球菌核酸酶已被用于研究中监测与 DNA 的杂交反应。它也被用于研究葡萄球菌核酸酶测定的最佳条件。

生化/生理作用

可水解DNA的5′-磷酸二酯键。

其他说明

注意:1μmole单位 = 约85 A260 单位,其中A260单位相当于在pH 8.8,37℃条件下,在3.55 mL反应体积中,30分钟内ΔA260为1.0。光路= 1cm。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

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分析证书(COA)

Lot/Batch Number

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Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Use of micrococcal nuclease to monitor hybridization reactions with DNA.
D L Kacian et al.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
Ho-Ryun Chung et al.
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
Henry Redel et al.
The Journal of infectious diseases, 207(7), 1105-1114 (2013-01-10)
Diabetic foot infections are a leading cause of lower extremity amputations. Our study examines the microbiota of diabetic skin prior to ulcer development or infection. In a case-control study, outpatient males were recruited at a veterans hospital. Subjects were swabbed
Ashish Kumar Singh et al.
Nature communications, 12(1), 7011-7011 (2021-12-03)
Numerous chromatin remodeling enzymes position nucleosomes in eukaryotic cells. Aside from these factors, transcription, DNA sequence, and statistical positioning of nucleosomes also shape the nucleosome landscape. The precise contributions of these processes remain unclear due to their functional redundancy in

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