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生物来源
Staphylococcus aureus
质量水平
表单
powder
比活
100-300 units/mg protein
组成
Protein, ≥40% E1%/280 (Balance primarily sodium acetate)
技术
DNA extraction: suitable
DNA purification: suitable
适用性
suitable for molecular biology
应用
cell analysis
储存温度
−20°C
基因信息
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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应用
微球菌核酸酶可用于消化染色质以鉴定核小体的位置。
来源于金黄色葡萄球菌的微球菌核酸酶已被用于研究中监测与 DNA 的杂交反应。它也被用于研究葡萄球菌核酸酶测定的最佳条件。
生化/生理作用
可水解DNA的5′-磷酸二酯键。
其他说明
注意：1μmole单位 = 约85 A260 单位，其中A260单位相当于在pH 8.8，37℃条件下，在3.55 mL反应体积中，30分钟内ΔA260为1.0。光路= 1cm。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Ho-Ryun Chung et al.
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
Use of micrococcal nuclease to monitor hybridization reactions with DNA.
D L Kacian et al.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Gang Wei et al.
Methods in enzymology, 513, 297-313 (2012-08-30)
Gene transcription can be regulated through alteration of chromatin structure, such as changes in nucleosome positioning and histone-modification patterns. Recent development of techniques based on the next-generation sequencing technology has allowed high-resolution analysis of genome-wide distribution of these chromatin features.
Julien Roche et al.
Biochemistry, 51(47), 9535-9546 (2012-11-03)
The folding of staphylococcal nuclease (SNase) is known to proceed via a major intermediate in which the central OB subdomain is folded and the C-terminal helical subdomain is disordered. To identify the structural and energetic determinants of this folding free
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