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生物来源
Staphylococcus aureus
质量水平
表单
powder
比活
100-300 units/mg protein
组成
Protein, ≥40% E1%/280 (Balance primarily sodium acetate)
技术
DNA extraction: suitable
DNA purification: suitable
适用性
suitable for molecular biology
应用
cell analysis
储存温度
−20°C
基因信息
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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应用
微球菌核酸酶可用于消化染色质以鉴定核小体的位置。
来源于金黄色葡萄球菌的微球菌核酸酶已被用于研究中监测与 DNA 的杂交反应。它也被用于研究葡萄球菌核酸酶测定的最佳条件。
生化/生理作用
可水解DNA的5′-磷酸二酯键。
其他说明
注意:1μmole单位 = 约85 A260 单位,其中A260单位相当于在pH 8.8,37℃条件下,在3.55 mL反应体积中,30分钟内ΔA260为1.0。光路= 1cm。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
从最新的版本中选择一种:
分析证书(COA)
Optimal conditions for assay of staphylococcal nuclease
Journal of Food Science, 42, 421-424 (1977)
Use of micrococcal nuclease to monitor hybridization reactions with DNA.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
The Journal of infectious diseases, 207(7), 1105-1114 (2013-01-10)
Diabetic foot infections are a leading cause of lower extremity amputations. Our study examines the microbiota of diabetic skin prior to ulcer development or infection. In a case-control study, outpatient males were recruited at a veterans hospital. Subjects were swabbed
Nature communications, 12(1), 7011-7011 (2021-12-03)
Numerous chromatin remodeling enzymes position nucleosomes in eukaryotic cells. Aside from these factors, transcription, DNA sequence, and statistical positioning of nucleosomes also shape the nucleosome landscape. The precise contributions of these processes remain unclear due to their functional redundancy in
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