N5386
微球菌核酸酶 来源于金黄色葡萄球菌
100-300 units/mg protein
别名:
MNase, 微球菌核酸内切酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-748-0
MDL number:
Specific activity:
100-300 units/mg protein
Biological source:
Staphylococcus aureus
产品名称
微球菌核酸酶 来源于金黄色葡萄球菌, 100-300 units/mg protein
biological source
Staphylococcus aureus
form
powder
specific activity
100-300 units/mg protein
composition
Protein, ≥40% E1%/280 (, Balance primarily sodium acetate)
technique(s)
DNA extraction: suitable
DNA purification: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
storage temp.
−20°C
Quality Level
Gene Information
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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Application
微球菌核酸酶可用于消化染色质以鉴定核小体的位置。
来源于金黄色葡萄球菌的微球菌核酸酶已被用于研究中监测与 DNA 的杂交反应。它也被用于研究葡萄球菌核酸酶测定的最佳条件。
Biochem/physiol Actions
可水解DNA的5′-磷酸二酯键。
Other Notes
注意：1μmole单位 = 约85 A260 单位，其中A260单位相当于在pH 8.8，37℃条件下，在3.55 mL反应体积中，30分钟内ΔA260为1.0。光路= 1cm。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Use of micrococcal nuclease to monitor hybridization reactions with DNA.
D L Kacian et al.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
Ho-Ryun Chung et al.
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
Frank J Hernandez et al.
Nature medicine, 20(3), 301-306 (2014-02-04)
Technologies that enable the rapid detection and localization of bacterial infections in living animals could address an unmet need for infectious disease diagnostics. We describe a molecular imaging approach for the specific, noninvasive detection of S. aureus based on the
Ashish Kumar Singh et al.
Nature communications, 12(1), 7011-7011 (2021-12-03)
Numerous chromatin remodeling enzymes position nucleosomes in eukaryotic cells. Aside from these factors, transcription, DNA sequence, and statistical positioning of nucleosomes also shape the nucleosome landscape. The precise contributions of these processes remain unclear due to their functional redundancy in
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