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重组
expressed in E. coli
质量水平
方案
≥85% (SDS-PAGE)
表单
buffered aqueous glycerol solution
enzyme activity
≥10 units/mg protein
分子量
protein apparent mol wt ~80 kDa
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... NEK2(4751)
应用
Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.
生化/生理作用
Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome.
单位定义
One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP
外形
Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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