N3393
萘酚蓝黑
BioReagent, suitable for electrophoresis
别名:
氨基黑 10B, 水牛黑色 NBR, 酸性黑1
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关于此项目
经验公式（希尔记法）:
C22H14N6Na2O9S2
化学文摘社编号:
分子量:
616.49
EC Number:
213-903-1
UNSPSC Code:
41105322
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Colour Index Number:
20470
Beilstein/REAXYS Number:
5374263
产品名称
萘酚蓝黑, BioReagent, suitable for electrophoresis
InChI
1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
InChI key
AOMZHDJXSYHPKS-DROYEMJCSA-L
SMILES string
[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
product line
BioReagent
form
powder
composition
Dye content, ~85%
technique(s)
protein staining: suitable
impurities
≤10% water
suitability
suitable for electrophoresis
Quality Level
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Application
萘酚蓝黑已被用于蛋白质印迹膜的染色，可用于检测转移至膜的所有蛋白质。
Biochem/physiol Actions
萘酚蓝黑染料可用于在聚丙烯酰胺凝胶、琼脂糖凝胶和硝酸纤维素膜上对蛋白质进行染色。电泳后，建议将蛋白固定在凝胶中。然后可以将凝胶用0.1％萘酚蓝黑在7％（v/v）的乙酸中染色至少2小时，并用7％（v/v）乙酸溶液脱色。检测灵敏度约为考马斯蓝R的20％。
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1B
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate.
E De Vito et al.
Experientia, 22(2), 124-125 (1966-02-15)
PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family.
Meyer D, et al.
Journal of Bacteriology, 188, 4903-4917 (2006)
Teresa M Lamb et al.
G3 (Bethesda, Md.), 3(12), 2273-2280 (2013-10-22)
Precise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled
Mary-Ann Glasier et al.
Current eye research, 33(8), 631-640 (2008-08-13)
To compare two variations of a membrane-based protein assay utilizing Amido black (AB) detection with a commercially available 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA) assay for use in the quantitation of individual tear proteins, pooled human tear proteins, and protein extracted from ex
Amit Parikh et al.
Biotechnology letters, 27(5), 323-326 (2005-04-19)
Cyanobacterial cultures isolated from sites polluted by industrial textile effluents were screened for their ability to decolorize cyclic azo dyes. Gloeocapsa pleurocapsoides and Phormidium ceylanicum decolorized Acid Red 97 and FF Sky Blue dyes by more than 80% after 26
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