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Merck
CN

N3393

Sigma-Aldrich

萘酚蓝黑

BioReagent, suitable for electrophoresis

别名:

氨基黑 10B, 水牛黑色 NBR, 酸性黑1

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About This Item

经验公式(希尔记法):
C22H14N6Na2O9S2
CAS号:
分子量:
616.49
颜色索引号:
20470
Beilstein:
5374263
EC 号:
MDL编号:
UNSPSC代码:
41105322
PubChem化学物质编号:
NACRES:
NA.32

产品线

BioReagent

质量水平

形式

powder

组成

Dye content, ~85%

技术

protein staining: suitable

杂质

≤10% water

适用性

suitable for electrophoresis

SMILES字符串

[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

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应用

萘酚蓝黑已被用于蛋白质印迹膜的染色,可用于检测转移至膜的所有蛋白质。

生化/生理作用

萘酚蓝黑染料可用于在聚丙烯酰胺凝胶、琼脂糖凝胶和硝酸纤维素膜上对蛋白质进行染色。电泳后,建议将蛋白固定在凝胶中。然后可以将凝胶用0.1%萘酚蓝黑在7%(v/v)的乙酸中染色至少2小时,并用7%(v/v)乙酸溶液脱色。检测灵敏度约为考马斯蓝R的20%。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Sens. 1B

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


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PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family.
Meyer D, et al.
Journal of Bacteriology, 188, 4903-4917 (2006)
Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate.
E De Vito et al.
Experientia, 22(2), 124-125 (1966-02-15)
Amit Parikh et al.
Biotechnology letters, 27(5), 323-326 (2005-04-19)
Cyanobacterial cultures isolated from sites polluted by industrial textile effluents were screened for their ability to decolorize cyclic azo dyes. Gloeocapsa pleurocapsoides and Phormidium ceylanicum decolorized Acid Red 97 and FF Sky Blue dyes by more than 80% after 26
Mary-Ann Glasier et al.
Current eye research, 33(8), 631-640 (2008-08-13)
To compare two variations of a membrane-based protein assay utilizing Amido black (AB) detection with a commercially available 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA) assay for use in the quantitation of individual tear proteins, pooled human tear proteins, and protein extracted from ex
Shih-Hsien Chang et al.
Journal of hazardous materials, 166(2-3), 1279-1288 (2009-01-23)
Degradation of Remazol Brilliant Blue R (anthraquinone dye) and Acid Black 1 (azo dye) by Fenton oxidation and low-cost Fe(0)/air process was compared. The doses of Fenton reagent needed for decolorization of Remazol Brilliant Blue R was much higher than

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