产品线
BioReagent
质量水平
形式
powder
组成
Dye content, ~85%
技术
protein staining: suitable
杂质
≤10% water
适用性
suitable for electrophoresis
SMILES字符串
[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
InChI
1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
InChI key
AOMZHDJXSYHPKS-DROYEMJCSA-L
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相关类别
应用
萘酚蓝黑已被用于蛋白质印迹膜的染色,可用于检测转移至膜的所有蛋白质。
生化/生理作用
萘酚蓝黑染料可用于在聚丙烯酰胺凝胶、琼脂糖凝胶和硝酸纤维素膜上对蛋白质进行染色。电泳后,建议将蛋白固定在凝胶中。然后可以将凝胶用0.1%萘酚蓝黑在7%(v/v)的乙酸中染色至少2小时,并用7%(v/v)乙酸溶液脱色。检测灵敏度约为考马斯蓝R的20%。
警示用语:
Warning
危险声明
危险分类
Skin Sens. 1B
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family.
Journal of Bacteriology, 188, 4903-4917 (2006)
Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate.
Experientia, 22(2), 124-125 (1966-02-15)
Biotechnology letters, 27(5), 323-326 (2005-04-19)
Cyanobacterial cultures isolated from sites polluted by industrial textile effluents were screened for their ability to decolorize cyclic azo dyes. Gloeocapsa pleurocapsoides and Phormidium ceylanicum decolorized Acid Red 97 and FF Sky Blue dyes by more than 80% after 26
Current eye research, 33(8), 631-640 (2008-08-13)
To compare two variations of a membrane-based protein assay utilizing Amido black (AB) detection with a commercially available 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA) assay for use in the quantitation of individual tear proteins, pooled human tear proteins, and protein extracted from ex
Journal of hazardous materials, 166(2-3), 1279-1288 (2009-01-23)
Degradation of Remazol Brilliant Blue R (anthraquinone dye) and Acid Black 1 (azo dye) by Fenton oxidation and low-cost Fe(0)/air process was compared. The doses of Fenton reagent needed for decolorization of Remazol Brilliant Blue R was much higher than
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