推荐产品
生物来源
Clostridium perfringens str. 13
质量水平
类型
Type VI
表单
lyophilized powder
比活
2-10 units/mg protein (mucin)
6-15 units/mg protein (using 4MU-NANA)
组成
Protein, ≥50% biuret
储存温度
−20°C
基因信息
Clostridium perfringens str. 13 ... nanI(988807)
正在寻找类似产品? 访问 产品对比指南
相关类别
一般描述
神经氨酸酶是水解酶，可促进流感病毒从感染细胞释放并促进病毒传播。
应用
产气荚膜梭菌 (C. welchii) 的神经氨酸酶已用于评估适合用作制备试验底物的糖蛋白组分的研究。在一项研究中也被用来研究一种ε毒素对 MDCK 细胞的作用。
生化/生理作用
产气荚膜梭菌的神经氨酸酶降低人白血病原始粒细胞的活力，减弱其激活淋巴细胞的能力。
使用神经氨酸酶去除细胞表面上糖蛋白的唾液酸残基已有多次报道。通常，实验步骤中会表明将神经氨酸酶在PBS中，37℃条件下孵育30分钟进行使用，然后用PBS洗涤几次。用浓度低得多的神经氨酸酶处理组织切片需要更长的温育时间：在pH 4.2-5的0.1M乙酸盐缓冲液中，对于1-4U/mL浓度，需要在37℃下孵育2-20小时。
利用神经氨酸酶裂解唾液酸基团已用来研究糖蛋白结构的抗体识别。与其他两种方法进行比较，使用神经氨酸酶可更好的对N-乙酰神经氨酸进行估测。
神经氨酸酶可用于从多种糖蛋白中切割末端N-乙酰神经氨酸（唾液酸）。 来自产气荚膜梭菌的酶可切割末端唾液酸残基，这些残基可通过α-2,3-、α-2,6-或α-2,8-与Gal、GlcNac、GalNAc、AcNeu、GlcNeu、寡糖、糖脂或糖蛋白进行连接。切割的相对速率按以下顺序逐渐降低：α-2-3>α-2-6。α-2-8。与来自A. ureafaciens的神经氨酸酶（Sigma N3642）（优先切割α-2-6连接的残基）相比，来自产气荚膜梭菌的神经氨酸酶可最有效地切割α-2-3连接的唾液酸残基。
制备说明
从 V 型 (N 2876) 色谱纯化
分析说明
按照4MU-NANA单位进行包装
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Ana León-Rodríguez et al.
Scientific reports, 12(1), 11581-11581 (2022-07-09)
Short-term behavioral alterations are associated with infection and aid the recovery from sickness. However, concerns have raised that sustained behavioral disturbances after acute neuroinflammation could relate to neurological diseases in the long run. We aimed to explore medium- and long-term
L Petit et al.
Journal of bacteriology, 179(20), 6480-6487 (1997-10-23)
Epsilon-toxin is produced by Clostridium perfringens types B and D and is responsible for a rapidly fatal enterotoxemia in animals, which is characterized by edema in several organs due to an increase in blood vessel permeability. The Madin-Darby canine kidney
Xiaomin Xi et al.
Cell death & disease, 13(8), 708-708 (2022-08-16)
Breast cancer, the most common cancer in women, usually exhibits intrinsic insensitivity to drugs, even without drug resistance. MUC1 is a highly glycosylated transmembrane protein, overexpressed in breast cancer, contributing to tumorigenesis and worse prognosis. However, the molecular mechanism between
A Ulloa-Aguirre et al.
Biology of reproduction, 30(2), 382-387 (1984-03-01)
Graded removal of sialic acid residues from a purified preparation of rat follicle-stimulating hormone (FSH; NIADDK-FSH-I-5) by neuraminidase digestion resulted in the production of FSH isohormones with isoelectric points identical to those found within pituitary tissue. In addition, each neuraminidase-produced
A G Fraser et al.
Journal of medical microbiology, 8(2), 235-249 (1975-05-01)
A glycoprotein fraction (fraction VII) suitable for use as a substrate in assays of microbial neuraminidase was prepared from pooled human plasma. It is pasteurised during preparation to eliminate the risk of transmission of serum hepatitis. This results in polymerisation
商品
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
实验方案
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
在测定神经氨酸酶活性时，使用了一种以N-乙酰新胺酸为酶底物的比色法进行测定。神经氨酸酶是一种可促进流感病毒从感染细胞中释放的水解酶。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门