应用
开发用作酶免疫分析 (EIA) 中检测碱性磷酸酶活性的可溶性底物。pNPP 是基于高灵敏度碱性磷酸酶的 EIA 检测中的首选底物。将 1 片 pNPP 片剂和 1 片 Tris 缓冲液片剂溶于去离子水中可作为即用型底物。各 Sigma 快速 pNPP 片剂组得到的溶液含 1.0 mg/mL pNPP，0.2 MTris 缓冲液。使用 pNPP 的 EIA 应用可在定时测定中读取，或用 3N NaOH 停止以延迟读取。SIGMA 快速 ™ pNPP仅需要添加蒸馏水或去离子水，即可制备活性底物溶液。片剂单独包装在铝箔包装中 。
重悬
将每片片剂溶于 20 mL 去离子水中，得到含 pNPP 的即用型缓冲液。
法律信息
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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