N2415
核苷磷酸化酶 细菌
recombinant, expressed in E. coli, ≥10 units/mg protein
别名:
PNP, 嘌呤核苷磷酸化酶, 嘌呤核苷：正磷酸核糖基转移酶
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关于此项目
化学文摘社编号:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-857-3
MDL number:
Specific activity:
≥10 units/mg protein
Assay:
≥80% protein basis (biuret)
Recombinant:
expressed in E. coli
SMILES string
[n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO
InChI
1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1
InChI key
MRWXACSTFXYYMV-FDDDBJFASA-N
recombinant
expressed in E. coli
assay
≥80% protein basis (biuret)
form
lyophilized powder
specific activity
≥10 units/mg protein
sequence note
MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
Escherichia coli ... deoD(945654)
Application
核苷磷酸化酶被用于偶联酶系统以测量蛋白质去磷酸化。
细菌核苷磷酸化酶已被用于反应缓冲液中，并已通过缓冲液交换去除残留的磷酸盐。
细菌核苷磷酸化酶已被用于鉴定并表征耻垢分枝杆菌中的两种腺苷磷酸化酶活性。细菌核苷磷酸化酶也已被用于研究3,5-二氯苯甲酰基取代的2-脱氧-D-核糖-1-磷酸衍生物对嘧啶和嘌呤核苷磷酸化酶的抑制作用。
Biochem/physiol Actions
催化下列反应：嘌呤核苷+磷酸盐=嘌呤+α-D-核糖1-磷酸
核苷磷酸化酶/嘌呤核苷磷酸化酶（PNP）是一种可参与嘌呤补救途径的酶。 它还有助于将肌苷可逆地裂解为次黄嘌呤，以及将鸟苷可逆地裂解为鸟嘌呤。PNP缺乏会导致一些神经系统表现。
Preparation Note
其是一种无盐和无动物成分的酶制剂。该酶应溶解在10mM KPO4（pH 7.4）溶液中。
Other Notes
一个酶活性单位是指在pH 7.4、25°C时，每分钟使 1.0微摩尔肌苷发生磷酸分解而形成次黄嘌呤和核糖-1-磷酸所需的酶量。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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