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重组
expressed in CHO cells
质量水平
描述
contains a C-terminal 6-His tag
方案
≥90% (SDS-PAGE)
表单
solution
enzyme activity
≥15 units/mg protein
分子量
~61 kDa by SDS-PAGE (reducing)
包装
vial of 6-12 μg
杂质
≤1.0 EU/μg endotoxin
NCBI登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... NT5E(4907)
一般描述
5′-核苷酸酶是广泛存在于哺乳动物细胞中的胞外酶。有助于从 5′ 加速磷酸基的水解反应核苷酸，产生相应的核苷。
生化/生理作用
5′-核苷酸酶 (nucleotidase) 又称 CD73，是一种能转化核苷-5′−单磷酸生成核苷，底物偏好 AMP。天然 5'-核苷酸酶是一种 GPI 锚定蛋白，其暴露量因缺氧而上调。5′-核苷酸酶在体内有许多功能 ，包括产生细胞外腺苷。
5′-核苷酸酶具有多种临床意义。在体外肿瘤血管生成和体内肿瘤免疫逃逸中，它是调控肿瘤细胞增殖、迁移和侵袭的关键分子。由于这一重要角色，该酶是癌症研究的潜在靶点。也参与细胞外核苷酸的补救，并在组织稳态的控制中起关键作用。
单位定义
一个单元将-37℃、pH 9.0 时每分钟从单磷酸盐水解 1.0μmol腺苷 5′-无机磷。
外形
提供含 Tris、NaCl、CaCl 2、 和 20% 甘油的溶液。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Anna Wilk et al.
Scientific reports, 10(1), 651-651 (2020-01-22)
Changes in nicotinamide adenine dinucleotide (NAD+) levels that compromise mitochondrial function trigger release of DNA damaging reactive oxygen species. NAD+ levels also affect DNA repair capacity as NAD+ is a substrate for PARP-enzymes (mono/poly-ADP-ribosylation) and sirtuins (deacetylation). The ecto-5'-nucleotidase CD73
Zhao-wei Gao et al.
BioMed research international, 2014, 460654-460654 (2014-08-16)
Purinergic signaling has emerged as an important player in cancer progression and is regulated by a series of nucleotidases. Among the enzyme cascade, CD73, which catelyzes AMP breakdown to adenosine, has been found to be overexpressed in many types of
Isolation of a plasma-membrane subfraction from rat liver containing an insulin-sensitive cyclic-AMP phosphodiesterase.
P D House et al.
European journal of biochemistry, 24(3), 429-437 (1972-01-21)
5'-Nucleotidase: molecular structure and functional aspects.
H Zimmermann
The Biochemical journal, 285 ( Pt 2), 345-365 (1992-07-15)
Bertrand Allard et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 19(20), 5626-5635 (2013-08-29)
Monoclonal antibodies (mAb) that block programmed death (PD)-1 or cytotoxic T lymphocyte antigen (CTLA-4) receptors have been associated with durable clinical responses against a variety of cancer types and hold great potential as novel cancer therapeutics. Recent evidence suggest that
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