描述
zwitterionic
检测方案
≥97% (TLC)
形式
solid
分子量
221.32 g/mol
颜色
white
溶解性
H2O: 50 mg/mL, clear, colorless
一般描述
NDSB 221 is a nondetergent sulfobetaine that has been found to improve unfolding reversibility.
应用
NDSB 221 has been used in a study to identify a novel ligand binding site in phosphoserine phosphatase from Thermococcus onnurineus. It has also been used in a study to investigate its effects on acidic fibroblast growth factor (aFGF).
Non-detergent sulfobetaine is a compound used for non-denaturing protien purification. Increases the extraction yield of membrane, nuclear and cytoskeletal associated proteins. Zwitterionic over a wide pH range, easily removed by dialysis and no significant absorption in the near UV range. Specific applications include microsomal protein extraction, nuclear protein recovery, precipitation reduction in IEF, and membrane-bound protease purification. The typical usage concentration is 0.5 - 2.0 M.
其他说明
This product is non-micelle forming.
警示用语：
Danger
危险声明
危险分类
Skin Corr. 1B
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
A nondetergent sulfobetaine improves protein unfolding reversibility in microcalorimetric studies
Salvino S D'Amico and Georges G Feller
Analytical Biochemistry, 385, 3-3 (2009)
Identification of a novel ligand binding site in phosphoserine phosphatase from the hyperthermophilic archaeon Thermococcus onnurineus
Tae-Yang Jung et al.
Proteins: Structure, Function, and Genetics, 819-829 (2012)
Long Xiang et al.
Journal of magnetic resonance (San Diego, Calif. : 1997), 194(1), 147-151 (2008-07-12)
Prevention of aggregation is critical for analyzing protein structure. Non-detergent sulfobetaines (NDSBs) are known to prevent protein aggregation, but the molecular mechanisms of their anti-aggregation effect are poorly understood. To elucidate the underlying mechanisms, we analyzed the effects of dimethylethylammonium
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