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应用
癸酸 4-硝基苯酯可作为底物,以评估重组灰霉青霉 T55 菌株产生的羧酸酯酶。它也已用于肝胰腺提取物的一般酯酶活性测定。
癸酸 4-硝基苯酯已用作分光光度法评估脂肪酶活性的底物。它也已被用作估计羧酸酯酶活性的底物。
生化/生理作用
癸酸 4-硝基苯酯是脂肪酶的底物。与其他 4-硝基苯酯相比,胞外脂肪酶与 4-硝基苯基癸酸酯的水解反应速度更高。
Isolation and characterization of an enzyme from the Greenshell? mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid
Toxicon, 60(3), 406-419 (2012)
Prikladnaia biokhimiia i mikrobiologiia, 48(4), 376-382 (2012-10-06)
As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally
Methods in Molecular biology: Platelets and Megakaryocytes, volume 1, functional assays, 27-27 (2012)
Frontiers in bioengineering and biotechnology, 8, 844-844 (2020-08-28)
The multiple inhibitors tolerance of microorganism is important in bioconversion of lignocellulosic biomass which is a promising renewable and sustainable source for biofuels and other chemicals. The disruption of an unknown α/β hydrolase, which was termed KmYME and located in
Carboxyl ester hydrolase from Penicillium expansum: cloning, characterization and overproduction by Penicillium griseoroseum
Journal of Applied Microbiology, 115(1), 114-124 (2013)
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