N0252
对硝基苯酚癸
lipase substrate, ≥98% (TLC), powder
别名:
4-硝基苯基癸酸酯, 癸酸4-硝基苯酯
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关于此项目
经验公式（希尔记法）:
C16H23NO4
化学文摘社编号:
分子量:
293.36
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
1915230
产品名称
对硝基苯酚癸, lipase substrate
InChI
1S/C16H23NO4/c1-2-3-4-5-6-7-8-9-16(18)21-15-12-10-14(11-13-15)17(19)20/h10-13H,2-9H2,1H3
SMILES string
CCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI key
RRNKCSIEGPOIKI-UHFFFAOYSA-N
assay
≥98% (TLC)
form
powder
solubility
chloroform: soluble 100 mg/mL, colorless to faintly yellow
storage temp.
−20°C
Quality Level
Application
癸酸 4-硝基苯酯可作为底物，以评估重组灰霉青霉 T55 菌株产生的羧酸酯酶。它也已用于肝胰腺提取物的一般酯酶活性测定。
癸酸 4-硝基苯酯已用作分光光度法评估脂肪酶活性的底物。它也已被用作估计羧酸酯酶活性的底物。
Biochem/physiol Actions
癸酸 4-硝基苯酯是脂肪酶的底物。与其他 4-硝基苯酯相比，胞外脂肪酶与 4-硝基苯基癸酸酯的水解反应速度更高。
Isolation and characterization of an enzyme from the Greenshell? mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid
MacKenzie LA, et al.
Toxicon, 60(3), 406-419 (2012)
Carboxyl ester hydrolase from Penicillium expansum: cloning, characterization and overproduction by Penicillium griseoroseum
Correa TLR, et al.
Journal of Applied Microbiology, 115(1), 114-124 (2013)
V M Gumerov et al.
Prikladnaia biokhimiia i mikrobiologiia, 48(4), 376-382 (2012-10-06)
As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally
Methods in Molecular biology: Platelets and Megakaryocytes, volume 1, functional assays, 27-27 (2012)
Janaina Marques de Almeida et al.
Scientific reports, 8(1), 10000-10000 (2018-07-04)
We determined the effect of the His-tag on the structure, activity, stability and immobilization of LipC12, a highly active lipase from a metagenomic library. We purified LipC12 with a N-terminal His-tag and then removed the tag using tobacco etch virus
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