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生物来源
human liver (hepatocytes)
表单
liquid
用途
sufficient for 1 96-well plate(s) (or 24-well plate)
包装
vial of ≥5 mil. cells
制造商/商品名称
LifeNet Health®
生长模式
adherent
描述
≥70%, confluent monolayer up to day 4
技术
cell culture | mammalian: suitable
drug transporter assay: suitable
genotyping: suitable
应用
ADME/TOX
相关疾病
non-alcoholic steatohepatitis (NASH); non-alcoholic/simple fatty liver (NAFL)
运输
liquid nitrogen
储存温度
−196°C
一般描述
We provide high viability cryoplatable hepatocytes, sourced from LifeNet Health® that predictably represents in vivo conditions. Applications for primary hepatocytes include in vitro evaluation of metabolism, drug-drug interaction, drug transporter activity, and toxicity of drug candidates. There are many other applications that encompass cryoplatable hepatocytes.
应用
See technical bulletins for detailed protocols.
Click lot availability link to get a complete list of currently available hepatocyte lots from LifeNet Health®.
Once the lot number(s) of interest are determined enter the lot number into the “Certificate of Analysis” request search engine at the bottom of the page to obtain a pdf copy of the CoA. Note, lot number CoA upload is only available on the product number detail page corresponding with the lot number.
Click lot availability link to get a complete list of currently available hepatocyte lots from LifeNet Health®.
Once the lot number(s) of interest are determined enter the lot number into the “Certificate of Analysis” request search engine at the bottom of the page to obtain a pdf copy of the CoA. Note, lot number CoA upload is only available on the product number detail page corresponding with the lot number.
特点和优势
These cryoplatable hepatocytes are sourced from LifeNet Health®. All cryoplatable hepatocytes have post-thaw viabilities over 80%. These lots are also characterized for Phase I CYP450 isoform and Phase II drug-metabolizing enzyme activity. Cryoplatable hepatocytes can be used for a variety of applications including metabolic stability, clearance, metabolite ID, metabolite profiling, species comparison studies, and uptake transporter studies.
Each lot is fully characterized for viability/stability, cell yield, attachment efficiency, optimal seeding density, monolayer confluency, Phase I and Phase II drug metabolizing enzyme activities and cyp induction. We have a wide selection of lots that can be used for a variety of applications, including, but not limited to:
Each lot is fully characterized for viability/stability, cell yield, attachment efficiency, optimal seeding density, monolayer confluency, Phase I and Phase II drug metabolizing enzyme activities and cyp induction. We have a wide selection of lots that can be used for a variety of applications, including, but not limited to:
- Drug metabolizing enzyme activity (stability, clearance, metabolite profiling and ID)
- Time-dependent metabolism (plated assay for low-clearance compounds)
- CYP450 Induction (mRNA and activity)
- Plated drug transporter assays (uptake and efflux)
- Toxicity assays
- High content analysis
- High content imaging
- 3D culture
包装
This product will be shipped in a liquid nitrogen dry shipper supplied from Cryoport. Please remember to ship the container back to Cryoport once you have removed the materials. Additional Cryoport shipping charges may apply.
Please see the Cryoport website for more details on the dry shipper.
Please see the Cryoport website for more details on the dry shipper.
法律信息
LifeNet Health is a registered trademark of LifeNet Health
自备
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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