HepaRG^™ 5F Control Cells

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

This product consists of ZFN engineered HepaRG 5F clone control cells. They are intended for use as both control cells for HepaRG^™ knockout cells as well as for a wide variety of liver cell based assays. Hepa RG cells display hepatocyte-like functions and can be a replacement to primary human hepatocytes in a number of drug metabolism, disposition and genotoxicity studies.

HepaRG^™ 5F Control Cells has been used in a gene expression assay to study the effect of pregnane X receptor on the induction of human cytochrome P450 3A4 by the irreversible myeloperoxidase inactivator PF-06282999.

See technical bulletins for detailed protocols

Sigma′s HepaRG 5F Clone exhibits improved growth characteristics and enhanced functionality, expressing a large panel of drug metabolism enzymes; including cytochrome P450 enzymes CYP1A2, CYP2B6, CYP2C8/9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4, nuclear receptors AhR, PXR, CAR, LXR, and FXR, and phase II enzymes UGT, SULT, NAT and GST.

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.

HepaRG is a trademark of BioPredic International company

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.