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Merck
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安全信息

MSKT0003

Sigma-Aldrich

IA-MS Immunoaffinity Mass Spectrometry Kit - Streptavidin

96 Well Strip Plate with SOLu-Trypsin

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别名:
Target protein enrichment and quantification from complex background by mass spectrometry
UNSPSC代码:
41115859
NACRES:
NA.41

形式

solid

适用性

suitable for mass spectrometry

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

The Streptavidin IA-MS Immunoaffinity Mass Spectrometry Kit is a 96-well platform enabling robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS. Users provide their own biotinylated capture molecule and internal standard (available separately, internal standards may be SIL peptides or preferably full length SIL protein) and the kit contains all other components needed for in-well enrichment and rapid trypsin digestion of samples. The plate format consists of 8-well strips allowing for partial runs, and avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. The entire workflow can be performed in under 5 hours, resulting in digests ready for injection with no SPE or other cleanup required.

应用

Target protein enrichment and quantification from complex background by mass spectrometry.

特点和优势

  • Entire workflow including digest completed in under 5 hours, such that samples are injection ready at the end of the work day
  • 96-well plate format has potential for automation in a liquid-handling system
  • Bead-free system circumvents messy clean-up and eliminates risk of column clogging
  • Plate strips allow user to run as many or as few samples as desired

包装

Box with foam insert containing kit components.

组分

Streptavidin High Binding Capacity Coated Plate S2577-1EA
SOLu-Trypsin EMS0004-100UG
MS Denaturation Solution EMS00010-10ML
Rapid Trypsin Digestion Buffer EMS0009-30ML
Tris Buffered Saline with TWEEN® 20 (TBST)powder, pH 8.0 T9039
Tris Buffered Saline (TBS) powder, pH 8.0 T6664
EZ-Pierce™ plate seal Z721581-1EA

See datasheet for more information.

储存及稳定性

2 years when stored at 2-8 °C.

Related Presentation

法律信息

EZ-Pierce is a trademark of Excel Scientific, Inc.
TWEEN is a registered trademark of Croda International PLC

仅试剂盒组分

产品编号
说明
  • Rapid Trypsin Digestion Buffer 30 mL
  • MS Denaturation Solution

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • S2577Streptavidin High Capacity Coated Plates, 8 well strip, clear 1 plate化学品安全说明书
  • EMS0004SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid 4 ´ 100 mL (1 mg/mL)化学品安全说明书
  • S2577Streptavidin High Capacity Coated Plates, 8 well strip, clear 1 packet化学品安全说明书
  • T6664Tris Buffered Saline, pH 8.0, powder化学品安全说明书
  • Z721581EZ-Pierce™ films, Excel Scientific EZP-100, non-sterile 4 films化学品安全说明书

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 3 - STOT SE 1

储存分类代码

3 - Flammable liquids

WGK

WGK 3

闪点(°F)

75.2 °F

闪点(°C)

24 °C

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