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安全信息

MIRAP00182

Sigma-Aldrich

MystiCq® microRNA qPCR Assay Primer

hsa-miR-146a-5p

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UNSPSC代码:
41106308
NACRES:
NA.55

形式

liquid

浓度

10 mg/mL

成熟序列

UGAGAACUGAAUUCCAUGGGUU

mp

~76 °C

Sanger mature/minor 登记号

运输

wet ice

储存温度

−20°C

基因信息

一般描述

The MystiCq® microRNA qPCR Assay Primer is an integral part of the MystiCq microRNA qPCR Assay System. It has been designed to target specific microRNAs with the MystiCq Universal PCR primer and the MystiCq® microRNA SYBR® Green qPCR ReadyMix on cDNA templates generated by the MystiCq microRNA cDNA Synthesis Mix kit.

特点和优势

  • Proprietary design features of assay primers allow for specificity towards mature microRNAs vs. precursor-microRNAs
  • Extensive design and testing of oligo-dT adapter primer to incorporate complementary Universal PCR Primer sequence
  • No self-complementarity or primer dimer artifacts with the Universal PCR Primer
  • Optimized primer Tm designed to match the Universal PCR Primer
  • Universal cycling conditions ensures robust amplification for all assays in profiling experiments
  • Optimized PCR product Tm (75-78° C)

其他说明

Formerly hsa-miR-146a. Compatible with mmu-miR-146a and rno-miR-146a.

法律信息

MystiCq is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Maryam Sadegh Shesh Poli et al.
Cancer management and research, 12, 973-980 (2020-02-28)
MicroRNAs including miR146a have a regulatory role on the expression of genes and act with binding to 3'-UTR region of the genes. Cyclooxygenase-2 (COX-2) is involved in carcinogenesis as an inflammatory marker, and microRNA-146a (miR-146a) as a negative regulatory factor.

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