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质量水平
检测方法
fluorometric
相关疾病
cancer; immunological diseases; gastrointestinal diseases
运输
wet ice
储存温度
−20°C
一般描述
Diamine oxidase (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest levels are observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT.
特点和优势
- Linear detection range 0.5 to 6 U/L .
- Compatible with high-throughput handling systems.
适用性
The kit is suitable for diamine oxidase activity in serum or plasma samples.
原理
The Diamine Oxidase Assay Kit is based on the oxidation of putrescine to pyrroline plus NH3 and H2O2. The generated H2O2 is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at λEx = 530 nm/λEm = 585 nm is directly proportional to the enzyme activity.
WGK
WGK 1
法规信息
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