Myeloperoxidase (MPO) Assay Kit

Myeloperoxidase (MPO) is a peroxidase enzyme and can be found in neutrophils, monocytes, and some soft tissue macrophages. MPO has an ability to use chloride as a cosubstrate with hydrogen peroxide to generate hypochlorous acid, a powerful antimicrobial agent produced by neutrophils. However, an excessive production of hypochlorous acid can lead to oxidative stress and tissue damage. Inflammation may also result when MPO oxidizes various substances such as phenols and anilines. Studies show that increased MPO levels may increase the risk of myocardial infarction and cardiovascular disease.

The kit is suitable for myeloperoxidase (MPO) peroxidation activity determination in biological samples (e.g. cell lysates, tissues, etc.).

The Myeloperoxidase (MPO) Assay Kit is based on the MPO enzyme reaction with hydrogen peroxide (H2O2) which oxidizes the dye reagent to a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm , is proportional to the total peroxidation activity in the sample. The provided MPO inhibitor is used to suppress peroxidase activity due to MPO in order to differentiate other peroxidase activities that may be present in the sample.