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Merck
CN

MAK515

Sigma-Aldrich

Oxaloacetate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

别名:

Oxaloacetate Quantification Kit

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

质量水平

检测方法

colorimetric
fluorometric

相关疾病

cancer; neurological disorders

运输

wet ice

储存温度

−20°C

一般描述

Oxaloacetate (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO2 with pyruvate or phosphoenolpyruvate.

特点和优势

  • Linear detection range: 7 to 400 µM for the colorimetric assay and 1 to 40 µM for the fluorometric assay.

适用性

The kit is suitable for oxaloacetate determination in plasma, serum, tissue and culture media.

原理

The Oxaloacetate Assay Kit provides a simple, direct and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the oxaloacetate concentration in the sample.

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 1

法规信息

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