质量水平
检测方法
colorimetric
相关疾病
Alzheimer′s disease; neurological disorders; neurodegenerative disorders
运输
wet ice
储存温度
−20°C
一般描述
Malate dehydrogenase (MDH) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 is found in the cytoplasm and plays a key part in the malateaspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer′s disease.
特点和优势
- Linear detection range: 0.5 to 65 U/L
- Compatible with high-throughput handling systems.
适用性
The kit is suitable for malate dehydrogenase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)
原理
The Malate Dehydrogenase Assay Kit is a non-radioactive, colorimetric MDH assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
警示用语：
Danger
危险分类
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system
储存分类代码
3 - Flammable liquids
WGK
WGK 3
法规信息
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