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Merck
CN

MAK501

Sigma-Aldrich

Glucose Oxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

别名:

Glucose Oxidase Assay Kit

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

质量水平

应用

food and beverages
pharmaceutical

检测方法

colorimetric
fluorometric

相关疾病

endocrinological disorders, diabetes

运输

wet ice

储存温度

−20°C

一般描述

Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients.

特点和优势

  • Linear detection range: 0.02 to 10 U/L glucose oxidase for the colorimetric assay and 0.002 to 1.5 U/L for the fluorometric assay.

适用性

The kit is suitable for determining glucose oxidase activity in cell lysate, culture medium and other biological samples, as well as for studying the effects of drugs on glucose metabolism.

原理

The Glucose Oxidase Assay Kit uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to glucose oxidase activity in the sample.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

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