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Merck
CN

MAK498

Sigma-Aldrich

Alcohol Dehydrogenase (ADH) Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.05

质量水平

检测方法

colorimetric

相关疾病

gastrointestinal diseases

运输

wet ice

储存温度

−20°C

一般描述

Alcohol dehydrogenase (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation.

特点和优势

  • Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
  • Compatible with high-throughput handling systems.

适用性

The kit is suitable for alcohol dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

原理

The non-radioactive, colorimetric Alcohol Dehydrogenase (ADH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

警示用语:

Danger

危险分类

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

3 - Flammable liquids

WGK

WGK 2

法规信息

危险化学品
含少量动物源组分生物产品
常规特殊物品

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