一般描述
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.
特点和优势
- The linear detection range of the kit is 0.1 µM to 10 µM NADP+ /NADPH
- Compatible with high-throughput handling systems.
- Convenient, the procedure involves adding a single working reagent, and reading the optical density at time zero and 30 minutes at room temperature.
适用性
The kit is suitable for detection of NADP+ /NADPH concentrations and ratios in cell or tissue extracts.
原理
The NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ /NADPH and is not interfered by NAD+ /NADH.
警示用语：
Danger
危险分类
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
3 - Flammable liquids
WGK
WGK 2
法规信息
常规特殊物品
含少量动物源组分生物产品
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