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Merck
CN

MAK475

Sigma-Aldrich

Creatinine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC代码:
12352204
NACRES:
NA.84

质量水平

检测方法

colorimetric
fluorometric

相关疾病

renal disease; neurological disorders; genitourinary disorders

运输

wet ice

储存温度

−20°C

一般描述

Creatinine is synthesized in the body from creatine, which is produced from creatine phosphate during muscle contractions. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and subsequent creatin levels in urine are diminished. Creatinine testing is most widely used to assess kidney function.

特点和优势

  • The linear detection range of creatinine is 4.8 to 500 µM (0.054 - 5.7 mg/dL) for the colorimetric assay and 0.25 to 100 µM (0.0028 - 1.14 mg/dL) for the fluorometric assay.
  • Compatible with high-throughput handling systems.
  • Convenient. The procedure involves adding a single working reagent and reading after 60 minutes.

适用性

The kit is suitable for creatinine determination in urine, serum, plasma, and other biological preparations.

原理

The Creatinine Assay Kit uses a reaction sequence to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the creatinine concentration in the sample. The reaction specifically excludes both endogenous creatine and ammonia.

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象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

法规信息

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分析证书(COA)

Lot/Batch Number

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