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Merck
CN

MAK472

Sigma-Aldrich

Iron Assay Kit

sufficient for 250 colorimetric tests

别名:

Assay Kit for Iron Detection

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

质量水平

应用

food and beverages
pharmaceutical

检测方法

colorimetric

相关疾病

anemia; hepatitis; gastrointestinal diseases; hematological disorder

运输

ambient

储存温度

2-8°C

一般描述

Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with red blood cell destruction, decreased blood cell survival, acute hepatitis, certain sideroblastic anemias, ingestion of iron-rich diets, defects in iron storage (e.g., pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand on iron storage as during normal pregnancy.

特点和优势

  • Linear detection range 27 µg/dL (4.8 µM) to 1,000 µg/dL (179 µM) iron in 96-well plate assay.
  • Compatible with high-throughput handling systems.
  • The optimized formulation has greatly enhanced reagent and signal stability.
  • Low interference in biological samples.
  • No pretreatments are needed. Assays can be directly performed on serum samples

适用性

The kit is suitable for the detection of iron in biological samples such as serum and soil extracts, and for studying the effects of drugs on iron metabolism.

原理

The Iron Assay Kit is designed to measure total iron directly in serum without pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe2+. Fe3+ in the sample is reduced to Fe2+, allowing the assay to determine total iron concentration. The intensity of the color, measured at 590 nm, is directly proportional to the iron concentration in the sample.

象形图

Corrosion

警示用语:

Warning

危险声明

预防措施声明

危险分类

Met. Corr. 1

储存分类代码

8A - Combustible corrosive hazardous materials

WGK

WGK 1


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