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质量水平
储存条件
(No metal containers. Keep container tightly closed in a dry and well -ventilated place. Keep away from heat and sources of ignition.)
输入
tissue
cell culture medium
tissue
plasma
检测方法
colorimetric
相关疾病
endocrinological disorders, diabetes; hematological disorder
储存温度
−20°C
一般描述
Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.
应用
The Glucose Dehydrogenase Assay Kit may be used for:
- Metabolism Research
- Drug Discovery
特点和优势
- Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose dehydrogenase levels across a broad range from 0.5 to 200 U/L, providing both convenience and sensitivity.
- Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
适用性
The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).
原理
The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.
其他说明
For additional information on our range of Biochemicals, please complete this form.
警示用语：
Danger
危险分类
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
3 - Flammable liquids
法规信息
常规特殊物品
含少量动物源组分生物产品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
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