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安全信息

MAK450

Sigma-Aldrich

Glucose Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

别名:

GDH Activity Assay Kit

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选择尺寸

1 G
CN¥2,791.57
100 G
CN¥27,915.71

CN¥2,791.57

预计发货时间May 30, 2025详情

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选择尺寸

变更视图
1 G
CN¥2,791.57
100 G
CN¥27,915.71

About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

CN¥2,791.57

预计发货时间May 30, 2025详情

获取大包装报价

质量水平

100

储存条件

(No metal containers. Keep container tightly closed in a dry and well -ventilated place. Keep away from heat and sources of ignition.)

输入

tissue
cell culture medium
tissue
plasma

检测方法

colorimetric

相关疾病

endocrinological disorders, diabetes; hematological disorder

储存温度

−20°C

相关类别

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853044

Boc-His-OH

Fmoc-3,4-dehydro-Pro-OH Novabiochem®

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assay

≥97% (TLC)

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥85.0% (acidimetric), ≥98% (TLC), ≥98.0% (HPLC)

product line

Novabiochem®

product line

Novabiochem®

product line

Novabiochem®

product line

Novabiochem®

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

reaction suitability

reaction type: Boc solid-phase peptide synthesis

reaction suitability

reaction type: Boc solid-phase peptide synthesis

reaction suitability

reaction type: Boc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

一般描述

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

应用

The Glucose Dehydrogenase Assay Kit may be used for:
  • Metabolism Research
  • Drug Discovery

特点和优势

  • Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose dehydrogenase levels across a broad range from 0.5 to 200 U/L, providing both convenience and sensitivity.
  • Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

适用性

The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

原理

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

其他说明

For additional information on our range of Biochemicals, please complete this form.

警示用语：

Danger

危险分类

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

3 - Flammable liquids

法规信息

常规特殊物品
含少量动物源组分生物产品
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    450CE12A09
    450CE03A20
    450CE12A09
    450CE03A20
    450CD07A26

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