Methionine Adenosyltransferase Activity Assay Kit (Colorimetric)

Methionine Adenosyltransferases (MATs), also known as Adenosylmethionine Synthetases are a family of enzymes that synthesize S-adenosyl-L-methionine (SAMe) from L-methionine and ATP. SAMe is a primary biochemical alkylation agent (one of only two possible methyl group donors) and is also a vital metabolic precursor of the trans-sulfuration and polyamine synthesis (aminopropylation) pathways. All organisms express at least one MAT enzyme. In mammals, three isozymes of MAT have been identified. MAT1 and MAT3 isozymes are predominantly expressed in the liver, whereas MAT2A is expressed in most tissues. Upregulation of the MAT2A isozyme has been linked to several human diseases. MAT2A has become a popular drug target for novel cancer therapeutics as well as for hepatic fibrosis and non-alcoholic fatty liver disease.

The kit is suitable for the rapid assessment of MAT activity in human or animal soft tissue (i.e. brain, liver, lung, etc.) homogenates and cultured cell lysates (adherent or suspension cells) or heterologously expressed recombinant MAT preparations.

The Methionine Adenosyltransferase Activity Assay Kit enables the rapid measurement of MAT activity in complex biological matrices. The assay is based on the detection of pyrophosphate, which is generated stoichiometrically during the generation of SAMe. Pyrophosphate is enzymatically metabolized to an intermediate product, which reacts with the probe to form a stable chromophore that is detected by absorbance at A₅₇₀ nm.

Methionine Adenosyltransferase Activity Assay Kit:

• Compatible with high-throughput handling systems.
• Limit of quantification of 2 mU MAT activity per well.
• Homogeneous assay.
• Does not require complicated sample processing.