Proline Assay Kit (Fluorometric)

Proline is a proteogenic amino acid, which plays an important role in protein folding. In humans, proline can be synthesized from glutamate and arginine. Proline is one of the conditionally essential amino acids in humans. Proline is also produced by honeybees as they process nectar into honey. Thus, its content in honey is often used as an indicator of honey ripeness and sugar adulteration. For mature honey, 180 mg-proline/kg of honey is used as an international standard. Proline contents are measured for its biological importance in plants where it also serves as a stress marker. Unstressed plants contain approximately 0.5 μmol proline per gram of plant tissue while stressed plants can range up to 100 times more proline than the unstressed ones.

Proline Assay Kit:

• Compatible with high-throughput handling systems.
• Sensitive, detecting as low as 5 pmol of proline per well.

The kit is suitable for the measurement of proline in biological samples such as serum, honey and mammalian and plant tissue. The kit can also be used in the analysis of honey maturation and in assaying proline concentration as a stress factor in plants.

In this assay, proline is converted to an intermediate, which will further react with a probe to produce a strong fluorometric signal (λ_Ex = 535 nm/λ_Em = 587nm).