Pyridoxal 5′-phosphate (Vitamin B6) Fluorometric Assay Kit

Pyridoxal 5′-phosphate (PLP), the active form of Vitamin B6 is a cofactor in a variety of metabolic reactions including transamination, decarboxylation, racemization, etc. Vitamin B6 comprises of pyridoxine, pyridoxamine and pyridoxal, which during metabolism is converted to the enzymatically active form namely PLP. Vitamin B6 is necessary for the synthesis of neurotransmitters, hemoglobin in red blood cells, fat metabolism etc. Vitamin B6 deficiency can lead to nervous disorders, peripheral neuropathy, anemia, seizures etc.

Pyridoxal 5′-phosphate (Vitamin B6) Assay Kit:

• Compatible with high-throughput handling systems.
• Detects as low as 0.2 pmol/well of PLP in biological samples.

The kit is suitable for the measurement of PLP in biological samples (ex. serum and mammalian tissue) and beverages and for the analysis of Vitamin B6 intake on serum.

In this assay, PLP reacts with a PLP-dependent enzyme thereby converting the substrate into an intermediate, which will further react with a probe to produce a strong fluorometric signal (λ_Ex = 535 nm/λEm = 587 nm).