应用
pharmaceutical
检测方法
fluorometric
相关疾病
genitourinary disorders; neurological disorders
储存温度
−20°C
一般描述
碱性磷酸酶(ALP)检测试剂盒(MAK530)新品到货！碱性磷酸酶（ALP）在碱性缓冲液中催化磷酸酯的水解，并产生有机自由基和无机磷酸盐。碱性磷酸酶水平和活性的变化与肝脏和骨骼中的几种疾病有关。碱性磷酸酶也是ELISA分析中与二抗偶联的常用酶。
应用
- Fibroblast growth factor-4 maintains cellular viability while enhancing osteogenic differentiation of stem cell spheroids in part by regulating RUNX2 and BGLAP expression.: This study used the Alkaline Phosphatase Activity Fluorometric Assay Kit to measure osteogenic differentiation in stem cell spheroids. The results demonstrated that fibroblast growth factor-4 (FGF-4) enhances the expression of osteogenic markers RUNX2 and BGLAP, indicating improved cellular viability and differentiation (Son et al., 2020) (Son et al., 2020).
- Osteogenic Effect of Inducible Nitric Oxide Synthase (iNOS)-Loaded Mineralized Nanoparticles on Embryonic Stem Cells.: This research employed the Alkaline Phosphatase Activity Fluorometric Assay Kit to assess the osteogenic differentiation of embryonic stem cells treated with iNOS-loaded nanoparticles. The findings revealed significant enhancement in osteogenic activity, suggesting potential applications in regenerative medicine (Lee et al., 2018) (Lee et al., 2018).
- Calcium Channel Opening Rather than the Release of ATP Causes the Apoptosis of Osteoblasts Induced by Overloaded Mechanical Stimulation.: The study used the Alkaline Phosphatase Activity Fluorometric Assay Kit to investigate osteoblast apoptosis under mechanical stress. It was found that calcium channel opening is the primary cause of apoptosis, providing insights into bone health and disease mechanisms (Liu et al., 2017) (Liu et al., 2017).
特点和优势
碱性磷酸酶活性荧光测定试剂盒：
- 与高通量处理系统兼容。
- 简单的程序，在∼1小时内得到结果。
- 超灵敏，检测灵敏度为∼1 μU。
适用性
该试剂盒适用于血清、血浆、尿液、精液、细胞培养基、细胞和组织中碱性磷酸酶活性的测定。
原理
在碱性磷酸酶活性荧光测定试剂盒中，ALP裂解非荧光4-甲基伞形酮磷酸二钠盐（MUP）底物的磷酸基团，产生强烈的荧光信号（λEx = 360 nm/λEm = 440 nm）。
法规信息
新产品
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