Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric)

Phosphoenolpyruvate Carboxykinase (PEPCK) is an enzyme which belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PEPCK-C, also called PCK1) and mitochondrial form (PEPCK-M). PEPCK-C is a rate-controlling step in gluconeogenesis. Recent studies found abnormal concentrations of PEPCK in diabetic mice. Therefore, accurate measurement of Phosphoenolpyruvate Carboxykinase activity is valuable for both mechanistic and therapeutic studies.

Phosphoenolpyruvate Carboxykinase Activity Assay Kit:

• Compatible with high-throughput handling systems.
• Sensitive, detecting than 10 μU of Phosphoenolpyruvate Carboxykinase activity per sample.

The kit is suitable for the measurement of Phosphoenolpyruvate Carboxykinase activity in various samples including tissue (rat liver, kidney, heart etc.) and adherent/suspension cells (HeLa, Jurkat, HEK 293 cells etc.). As well as the analysis of the gluconeogenesis pathway.

In this assay, Phosphoenolpyruvate Carboxykinase is coupled with a set of enzymes that convert phosphoenolpyruvate and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and is converted generating a colorimetric signal (A₅₇₀ nm). The color intensity is directly proportional to the amount of active Phosphoenolpyruvate Carboxykinase present in samples.