Arginase I (ARG1) Inhibitor Screening Kit (Colorimetric)

Arginase is a manganese-containing enzyme which catalyzes the conversion of arginine into urea and ornithine, which is the final reaction in the urea cycle. Arginase I (ARG1) is the liver isoform of arginase. Recent studies have showed that ARG1 expression by mature myeloid cells in tumor environment as demonstrated in a 3LL murine lung carcinoma model causes L-Arginine depletion by tumor-associated myeloid cells (TAMC). L-arginine depletion suppresses immune-response against tumor cells due to inhibition to T-cell proliferation. In addition, the depletion of arginine increases the reactive nitrogen species (NOS) and reactive oxygen species (ROS), which, in consequence, induces T-cell apoptosis and supports antigenic cell proliferation.

The Arginase I (ARG1) Inhibitor Screening Kit:

• Compatible with high-throughput handling systems.

The kit is suitable for the screening for inhibitors of human arginase I (ARG1).

The Arginase I (ARG1) Inhibitor Screening Kit is designed for screening of ARG1 inhibitors. Two substituted-2-amino-6-hexanoic acids have been studied as arginase inhibitor and in this kit, Amino-2-Borono-6-Hexanoic Acid (ABH) is provided as a positive control. The ARG1 activity is monitored by the increase in absorbance readings (A₄₅₀nm), while potential inhibitors will cause a decrease of absorbance.