Cathepsin D Assay Kit

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

The Cathepsin-D Activity Assay kit:

• Is a simple one-step procedure with results in 1-2 hours.

The kit is suitable for the detection of Cathepsin-D activity in cell lysates.

The Cathepsin-D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-DR-NH2) labeled with 7-Methoxycoumarin-4-acetic acid (MCA). Cell lysates or other samples that contain cathepsin-D will cleave the synthetic substrate to release fluorescence, which can then easily be quantified fluorometrically at λ_Ex = 328 nm/λ_Em = 460nm.