Neprilysin Activity Assay Kit (Fluorometric)

Neprilysin, also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid β (A β) peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, and Alzheimer′s disease. NEP has also been used as a biological marker in a type of childhood leukemia. The detection of NEP in endometrial stromal cells has been proposed as a helpful tool in the diagnosis of endometriosis. NEP is currently a focus of major interest in cardiovascular and neurological research.

Compatible with high-throughput handling systems.

Suitable for the measurement of Neprilysin activity in tissue homogenates (lung, kidney, etc.), cell culture (adherent or suspension cells)  and purified enzyme.

The Neprilysin Activity Assay Kit utilizes the ability of an active NEP to cleave a synthetic substrate (Abz-based peptide) to release a free fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. The substrate is specific to NEP and can differentiate the NEP activity from Trypsin and other structurally similar zinc metalloproteinase in biological samples such as Angiotensin-Converting Enzymes (ACE1, ACE2), Endothelin Converting Enzymes (ECE1, ECE2). The assay kit is simple, specific and can detect as low as 20 μU/mg of NEP activity.