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Merck
CN

MAK335

Sigma-Aldrich

琥珀酸盐检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

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别名:
Succinate Quantification Kit
UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

检测方法

colorimetric
fluorometric

相关疾病

cardiovascular diseases; immunological diseases

储存温度

−20°C

一般描述

琥珀酸盐,即琥珀酸,可以在所有植物和动物组织中找到。它是柠檬酸循环的中间体,在细胞内能量生成中起重要作用。琥珀酸盐由于毒性低,被广泛用作食品、饮料、制药工业的矫味剂。

应用

The Succinate Assay Kit may be used for:
  • Diabetes Research
  • Food and Beverage Testing
  • Mitochondrial Disorder Research

特点和优势

Efficient and Precise: Detect Succinate levels ranging from 10 to 400 μM (colorimetric) and 2 to 40 μM (fluorimetric) in a 30 minute room temperature reaction, providing both convenience and sensitivity.


Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

适用性

适用于食品、饮料、农产品等生物样品中琥珀酸盐的测定。

原理

琥珀酸盐检测试剂盒提供了一种简单的一步法检测琥珀酸盐的方法。在该测定中,琥珀酸盐转化为丙酮酸盐,丙酮酸盐与特定试剂和染料反应形成有色产物。反应产物在570 nm处的颜色强度或在λex/em=530/585 nm处的荧光与样品中的琥珀酸盐浓度成正比。 比色法的线性检测范围为10 μM至400 μM,荧光法的线性检测范围为2至40 μM。

其他说明

For additional information on our range of Biochemicals, please complete this form.

WGK

WGK 3

法规信息

常规特殊物品
含少量动物源组分生物产品

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Sulagna Bhattacharya et al.
EBioMedicine, 68, 103418-103418 (2021-06-09)
We have previously described an evolutionarily selected Tibetan prolyl hydroxylase-2 (PHD2D4E;C127S) variant that degrades the hypoxia-inducible factor (HIFα) more efficiently and protects these highlanders from hypoxia-triggered elevation in haemoglobin concentration. High altitude is known to cause acute mountain sickness (AMS)
Nishith M Shrimali et al.
EBioMedicine, 73, 103672-103672 (2021-11-06)
Phospho-Akt1 (pAkt1) undergoes prolyl hydroxylation at Pro125 and Pro313 by the prolyl hydroxylase-2 (PHD2) in a reaction decarboxylating α-ketoglutarate (αKG). We investigated whether the αKG supplementation could inhibit Akt-mediated activation of platelets and monocytes, in vitro as well as in

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