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检测方法
colorimetric
相关疾病
endocrinological disorders, diabetes; gastrointestinal diseases
储存温度
−20°C
一般描述
Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.
特点和优势
Compatible with high-throughput handling systems.
适用性
Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media.
原理
Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media).
警示用语：
Warning
危险分类
Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
3 - Flammable liquids
闪点(°F)
75.2 °F - closed cup
闪点(°C)
24 °C - closed cup
法规信息
新产品
Pathophysiology of diabetic retinopathy.
Tarr J M, et al.
ISRN ophthalmology (2013)
Luciana F Brito et al.
Applied microbiology and biotechnology, 104(11), 5095-5106 (2020-04-11)
Gene repression using the endonucleolytically deactivated dCas9 protein and sgRNAs (CRISPR interference or CRISPRi) is a useful approach to study gene functions. Here, we established CRISPRi in Paenibacillus sonchi genomovar Riograndensis SBR5, a plant growth promoting bacterium. CRISPRi system with
The current state of serum biomarkers of hepatotoxicity.
Ozer J, et al.
Toxicology, 245(3), 194-205 (2008)
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