山梨醇脱氢酶检测试剂盒

山梨醇脱氢酶（SDH）是一种可以催化山梨醇和果糖发生互变的酶。血清SDH水平升高代表肝脏损伤；因此，SDH在肝脏疾病的诊断中具有重要作用，特别是结合转氨酶。SDH水平也可用于评估糖尿病并发症，如增生性糖尿病视网膜病变。

Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.

Compatible with high-throughput handling systems.

Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media.

Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media).