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检测方法
colorimetric
相关疾病
cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders
储存温度
2-8°C
一般描述
过氧化物（如过氧化氢H2O2）是分子氧代谢产生的活性氧类，可作为细胞内信使和氧化应激来源。细胞可通过多种机制产生过氧化氢，如嗜中性粒细胞和巨噬细胞（呼吸爆发）中由NOX介导的ROS形成过程，或在线粒体呼吸的电子泄漏过程中由超氧阴离子歧化酶生成。 异常生成的过氧化氢会引起细胞氧化损伤和一些疾病进程，如哮喘、动脉粥样硬化、骨质疏松和神经退行性病变。
应用
过氧化物检测试剂盒用于测量大鼠组织匀浆中的过氧化氢水平。
特点和优势
兼容高通量操作系统。
适用性
适用于多种生物样品（如血清、柠檬酸盐-血浆、尿液、细胞裂解液和培养基）中无需预处理的过氧化物浓度的定量测定。
原理
过氧化物检测试剂盒提供简单且高通量的适应性测定法，用于定量测定无需预处理的多种生物样品（如血清、柠檬酸盐-血浆、尿液、细胞裂解液和培养基）中的过氧化物浓度。该检测利用Fe3+ −二甲酚橙显色反应，样品中的过氧化物将Fe2+氧化为Fe3+，（与二甲酚橙）形成可通过比色法(585 nm)测定的紫色复合物，与过氧化物浓度成正比。优化后的配方可减少原始样品中其他物质的干扰。1 μM H2O2的浓度相当于34 ng/mL或34 ppb。该试剂盒的检测范围为0.2–30 μM (7–1,020 ng/mL)H2O2（96孔板形式）。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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