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检测方法
colorimetric
相关疾病
cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders
储存温度
2-8°C
一般描述
过氧化物（如过氧化氢H2O2）是分子氧代谢产生的活性氧类，可作为细胞内信使和氧化应激来源。细胞可通过多种机制产生过氧化氢，如嗜中性粒细胞和巨噬细胞（呼吸爆发）中由NOX介导的ROS形成过程，或在线粒体呼吸的电子泄漏过程中由超氧阴离子歧化酶生成。 异常生成的过氧化氢会引起细胞氧化损伤和一些疾病进程，如哮喘、动脉粥样硬化、骨质疏松和神经退行性病变。
应用
过氧化物检测试剂盒用于测量大鼠组织匀浆中的过氧化氢水平。
特点和优势
兼容高通量操作系统。
适用性
适用于多种生物样品（如血清、柠檬酸盐-血浆、尿液、细胞裂解液和培养基）中无需预处理的过氧化物浓度的定量测定。
原理
过氧化物检测试剂盒提供简单且高通量的适应性测定法，用于定量测定无需预处理的多种生物样品（如血清、柠檬酸盐-血浆、尿液、细胞裂解液和培养基）中的过氧化物浓度。该检测利用Fe3+ −二甲酚橙显色反应，样品中的过氧化物将Fe2+氧化为Fe3+，（与二甲酚橙）形成可通过比色法(585 nm)测定的紫色复合物，与过氧化物浓度成正比。优化后的配方可减少原始样品中其他物质的干扰。1 μM H2O2的浓度相当于34 ng/mL或34 ppb。该试剂盒的检测范围为0.2–30 μM (7–1,020 ng/mL)H2O2（96孔板形式）。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
储存分类代码
8B - Non-combustible corrosive hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Steffen Pabel et al.
Circulation research, 130(7), 994-1010 (2022-02-24)
Atrial fibrillation (AF) and heart failure often coexist, but their interaction is poorly understood. Clinical data indicate that the arrhythmic component of AF may contribute to left ventricular (LV) dysfunction. This study investigates the effects and molecular mechanisms of AF
Dhiman Chakravarty et al.
Bioresource technology, 293, 122013-122013 (2019-09-09)
Here, we show the utility of a cyanobacterial biomass for overproduction and easy downstream processing of the thermostable protein KatB (a Mn-catalase). The nitrogen-fixing blue-green alga, Anabaena, was bioengineered to overexpress the KatB protein (An-KatB). Interestingly, pure An-KatB could be
Role of Cystathionine Gamma Lyase in Diabetic Nephropathy.
Anter A F, et al.
Journal of Advanced Biomedical and Pharmaceutical Sciences, 1(1 ), 33-40 (2018)
Han Xie et al.
Redox biology, 53, 102340-102340 (2022-05-25)
Generation of reactive oxygen species (ROS) are possibly induced by the crosstalk between mitochondria and endoplasmic reticula, which is physiologically important in apoptosis. Cytochrome c (Cyt c) is believed to play a crucial role in such signaling pathway by interrupting
Jason D Plummer et al.
Frontiers in cell and developmental biology, 7, 301-301 (2019-12-19)
Methionine restriction (MR) is one of only a few dietary manipulations known to robustly extend healthspan in mammals. For example, rodents fed a methionine-restricted diet are up to 45% longer-lived than control-fed animals. Tantalizingly, ongoing studies suggest that humans could
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